Role of glycoprotein IbIalpha mobility in platelet function

Evertdina van der Wal, Sandra Verhoef, Rober EG Schutgens, Marjolein Peters, Yaping Wu, Jan-Willem N Akkerman

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14 Citations (Scopus)

Abstract

Incubation at 0A?C is known to expose I?-N-acetyl-D-glucosamine residues on glycoprotein (GP) IbI? inducing receptor clustering and I? MI? 2-mediated platelet destruction by macrophages. Here we show that incubation at 0/37A?C (4 hours at 0A?C, followed by 1 hour at 37A?C to mimic cold-storage and post-transfusion conditions) triggers a conformational change in the N-terminal flank (NTF, amino acids, aa 1-35) but not in aa 36-282 of GPIbI? as detected by antibody binding. Addition of the sugar N-acetyl-D-glucosamine (GN) inhibits responses induced by 0/37A?C. Incubation at 0A?C shifts GPIbI? from the membrane skeleton to the cytoskeleton. Different GPIbI? conformations have little effect on VWF/ristocetin-induced aggregation, but arrest of NTF change by GN interferes with agglutination and spreading on a VWF-coated surface under flow. Strikingly, incubation at 0/37A?C initiates thromboxane A 2 formation through a von Willebrand factor (VWF)-independent and GPIbI?-dependent mechanism, as confirmed in VWF- and GPIbI?-deficient platelets. We conclude that the NTF change induced by 0/37A?C incubation reflects clustering of GPIbI? supports VWF/ristocetin-induced agglutination and spreading and is sufficient to initiate platelet activation in the absence of VWF. A? Schattauer 2010.
Original languageEnglish
Pages (from-to)1033 - 1043
Number of pages11
JournalThrombosis and Haemostasis
Volume103
Issue number5
DOIs
Publication statusPublished - 2010

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