Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish

Frisca Frisca; Daniel Colquhoun; Yona Goldshmit; Minna-Liisa Anko; Alice Pebay; Jan Kaslin

doi:10.1038/srep37678

Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish

Frisca Frisca, Daniel Colquhoun, Yona Goldshmit, Minna-Liisa Anko, Alice Pebay, Jan Kaslin

Research output: Contribution to journal › Article › Research › peer-review

8 Citations (Scopus)

Abstract

Lysophosphatidic acid (LPA) is a unique bioactive lysophospholipid that induces pleiotropic effects in various cell types and organisms by acting on its specific receptors. LPA is mainly synthetised extracellularly by the ectonucleotide pyrophosphatase/phosphodiesterase 2/autotaxin (enpp2). Altered LPA signalling is associated with embryonic abnormalities, suggesting critical roles for LPA during development. However, the role of LPA signalling during early embryogenesis is not well established. We demonstrate that enpp2/LPA signalling in the early zebrafish embryo results in altered axis and midline formation, defects in left right (L-R) patterning, ciliogenesis of the Kupffer’s vesicle (KV), through the modulation of cell migration during gastrulation in a lpar1–3Rho/ROCK-dependant manner. Overall, this study demonstrates an essential role of enpp2/LPA signalling during early embryogenesis.

Original language	English
Article number	37678
Number of pages	11
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep37678
Publication status	Published - 24 Nov 2016

Keywords

embryology
lipid signalling

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10.1038/srep37678

19558426-oaFinal published version, 1.69 MB

Cite this

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title = "Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish",

abstract = "Lysophosphatidic acid (LPA) is a unique bioactive lysophospholipid that induces pleiotropic effects in various cell types and organisms by acting on its specific receptors. LPA is mainly synthetised extracellularly by the ectonucleotide pyrophosphatase/phosphodiesterase 2/autotaxin (enpp2). Altered LPA signalling is associated with embryonic abnormalities, suggesting critical roles for LPA during development. However, the role of LPA signalling during early embryogenesis is not well established. We demonstrate that enpp2/LPA signalling in the early zebrafish embryo results in altered axis and midline formation, defects in left right (L-R) patterning, ciliogenesis of the Kupffer{\textquoteright}s vesicle (KV), through the modulation of cell migration during gastrulation in a lpar1–3Rho/ROCK-dependant manner. Overall, this study demonstrates an essential role of enpp2/LPA signalling during early embryogenesis.",

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T1 - Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish

AU - Frisca, Frisca

AU - Colquhoun, Daniel

AU - Goldshmit, Yona

AU - Anko, Minna-Liisa

AU - Pebay, Alice

AU - Kaslin, Jan

PY - 2016/11/24

Y1 - 2016/11/24

N2 - Lysophosphatidic acid (LPA) is a unique bioactive lysophospholipid that induces pleiotropic effects in various cell types and organisms by acting on its specific receptors. LPA is mainly synthetised extracellularly by the ectonucleotide pyrophosphatase/phosphodiesterase 2/autotaxin (enpp2). Altered LPA signalling is associated with embryonic abnormalities, suggesting critical roles for LPA during development. However, the role of LPA signalling during early embryogenesis is not well established. We demonstrate that enpp2/LPA signalling in the early zebrafish embryo results in altered axis and midline formation, defects in left right (L-R) patterning, ciliogenesis of the Kupffer’s vesicle (KV), through the modulation of cell migration during gastrulation in a lpar1–3Rho/ROCK-dependant manner. Overall, this study demonstrates an essential role of enpp2/LPA signalling during early embryogenesis.

AB - Lysophosphatidic acid (LPA) is a unique bioactive lysophospholipid that induces pleiotropic effects in various cell types and organisms by acting on its specific receptors. LPA is mainly synthetised extracellularly by the ectonucleotide pyrophosphatase/phosphodiesterase 2/autotaxin (enpp2). Altered LPA signalling is associated with embryonic abnormalities, suggesting critical roles for LPA during development. However, the role of LPA signalling during early embryogenesis is not well established. We demonstrate that enpp2/LPA signalling in the early zebrafish embryo results in altered axis and midline formation, defects in left right (L-R) patterning, ciliogenesis of the Kupffer’s vesicle (KV), through the modulation of cell migration during gastrulation in a lpar1–3Rho/ROCK-dependant manner. Overall, this study demonstrates an essential role of enpp2/LPA signalling during early embryogenesis.

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KW - lipid signalling

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VL - 6

JO - Scientific Reports

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Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish

Abstract

Keywords

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The role of immune cells and the transcription factor Hmga1 in boosting neural regeneration

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Role of ectonucleotide pyrophosphatase/phosphodiesterase 2 in the midline axis formation of zebrafish

Abstract

Keywords

Access to Document

Other files and links

Projects

The role of immune cells and the transcription factor Hmga1 in boosting neural regeneration

Equipment

FishCore

Monash Micro Imaging (MMI)

Cite this