Role of adenosine receptors in regulating chemotaxis and cytokine production of plasmacytoid dendritic cells

Max Schnurr, Tracey Toy, Amanda Shin, Gunther Hartmann, Simon Rothenfusser, Julia Soellner, Ian D. Davis, Jonathan Cebon, Eugene Maraskovsky

Research output: Contribution to journalArticleResearchpeer-review

141 Citations (Scopus)

Abstract

Plasmacytoid dendritic cells (PDCs) are potent regulators of immune function and the major source of type I interferon (IFN) following viral infection. PDCs are found at sites of inflammation in allergic reactions, autoimmune disorders, and cancer, but the mechanisms leading to the recruitment of PDCs to these sites remain elusive. During inflammation, adenosine is released and functions as a signaling molecule via adenosine receptors. This study analyzes adenosine receptor expression and function in human PDCs. Adenosine was found to be a potent chemotactic stimulus for immature PDCs via an A1 receptor-mediated mechanism. The migratory response toward adenosine was comparable to that seen with CXCL12 (stromal-derived factor-1α [SDF-1α), the most potent chemotactic stimulus identified thus far for immature PDCs. Upon maturation, PDCs down-regulate the A 1 receptor, resulting in a loss of migratory function. In contrast, mature PDCs upregulate the A2a receptor, which is positively coupled to adenylyl cyclase and has been implicated in the down-regulation of DC cytokine-producing capacity. We show that in mature PDCs adenosine reduces interleukin-6 (IL-6), IL-12, and IFN-α production in response to CpG oligodeoxynucleotides (ODN). These findings indicate that adenosine may play a dual role in PDC-mediated immunity by initially recruiting immature PDCs to sites of inflammation and by subsequently limiting the extent of the inflammatory response induced by mature PDCs by inhibiting their cytokine-producing capacity.

Original languageEnglish
Pages (from-to)1391-1397
Number of pages7
JournalBlood
Volume103
Issue number4
DOIs
Publication statusPublished - 15 Feb 2004

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