RNA polymer translocation with single-walled carbon nanotubes

Qi Lu, Jessica M. Moore, George Huang, Andrew S. Mount, Apparao M. Rao, Lyndon L. Larcom, Pu Chun Ke

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251 Citations (Scopus)

Abstract

The translocation of single-walled carbon nanotubes (SWNTs) across MCF7 breast cancer cells was demonstrated with radioisotope labeling. Hybrids of SWNT-RNA polymer poly(rU) were formed through a nonspecific binding mechanism which could allow for the dissociation of the poly(rU) from the SWNTs upon delivery. The cellular uptake of the hybrids was examined by confocal fluorescence microscopy. Through cell growth and MTS (3-(4,5-dimethylthiazol-2- yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assays, we showed the negligible cytotoxicity of SWNTs (up to 0.5 mg/mL) to MCF7 cells.

Original languageEnglish
Pages (from-to)2473-2477
Number of pages5
JournalNano Letters
Volume4
Issue number12
DOIs
Publication statusPublished - 1 Dec 2004
Externally publishedYes

Cite this

Lu, Q., Moore, J. M., Huang, G., Mount, A. S., Rao, A. M., Larcom, L. L., & Ke, P. C. (2004). RNA polymer translocation with single-walled carbon nanotubes. Nano Letters, 4(12), 2473-2477. https://doi.org/10.1021/nl048326j