RNA polymer translocation with single-walled carbon nanotubes

Qi Lu; Jessica M. Moore; George Huang; Andrew S. Mount; Apparao M. Rao; Lyndon L. Larcom; Pu Chun Ke

doi:10.1021/nl048326j

RNA polymer translocation with single-walled carbon nanotubes

Qi Lu, Jessica M. Moore, George Huang, Andrew S. Mount, Apparao M. Rao, Lyndon L. Larcom, Pu Chun Ke

Research output: Contribution to journal › Article › Research › peer-review

272 Citations (Scopus)

Abstract

The translocation of single-walled carbon nanotubes (SWNTs) across MCF7 breast cancer cells was demonstrated with radioisotope labeling. Hybrids of SWNT-RNA polymer poly(rU) were formed through a nonspecific binding mechanism which could allow for the dissociation of the poly(rU) from the SWNTs upon delivery. The cellular uptake of the hybrids was examined by confocal fluorescence microscopy. Through cell growth and MTS (3-(4,5-dimethylthiazol-2- yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assays, we showed the negligible cytotoxicity of SWNTs (up to 0.5 mg/mL) to MCF7 cells.

Original language	English
Pages (from-to)	2473-2477
Number of pages	5
Journal	Nano Letters
Volume	4
Issue number	12
DOIs	https://doi.org/10.1021/nl048326j
Publication status	Published - 1 Dec 2004
Externally published	Yes

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10.1021/nl048326j

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title = "RNA polymer translocation with single-walled carbon nanotubes",

abstract = "The translocation of single-walled carbon nanotubes (SWNTs) across MCF7 breast cancer cells was demonstrated with radioisotope labeling. Hybrids of SWNT-RNA polymer poly(rU) were formed through a nonspecific binding mechanism which could allow for the dissociation of the poly(rU) from the SWNTs upon delivery. The cellular uptake of the hybrids was examined by confocal fluorescence microscopy. Through cell growth and MTS (3-(4,5-dimethylthiazol-2- yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assays, we showed the negligible cytotoxicity of SWNTs (up to 0.5 mg/mL) to MCF7 cells.",

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T1 - RNA polymer translocation with single-walled carbon nanotubes

AU - Lu, Qi

AU - Moore, Jessica M.

AU - Huang, George

AU - Mount, Andrew S.

AU - Rao, Apparao M.

AU - Larcom, Lyndon L.

AU - Ke, Pu Chun

PY - 2004/12/1

Y1 - 2004/12/1

N2 - The translocation of single-walled carbon nanotubes (SWNTs) across MCF7 breast cancer cells was demonstrated with radioisotope labeling. Hybrids of SWNT-RNA polymer poly(rU) were formed through a nonspecific binding mechanism which could allow for the dissociation of the poly(rU) from the SWNTs upon delivery. The cellular uptake of the hybrids was examined by confocal fluorescence microscopy. Through cell growth and MTS (3-(4,5-dimethylthiazol-2- yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assays, we showed the negligible cytotoxicity of SWNTs (up to 0.5 mg/mL) to MCF7 cells.

AB - The translocation of single-walled carbon nanotubes (SWNTs) across MCF7 breast cancer cells was demonstrated with radioisotope labeling. Hybrids of SWNT-RNA polymer poly(rU) were formed through a nonspecific binding mechanism which could allow for the dissociation of the poly(rU) from the SWNTs upon delivery. The cellular uptake of the hybrids was examined by confocal fluorescence microscopy. Through cell growth and MTS (3-(4,5-dimethylthiazol-2- yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assays, we showed the negligible cytotoxicity of SWNTs (up to 0.5 mg/mL) to MCF7 cells.

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SP - 2473

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JO - Nano Letters

JF - Nano Letters

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ER -

RNA polymer translocation with single-walled carbon nanotubes

Abstract

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