@inbook{8f0e9871d1c04777b70de6af15c97966,
title = "RNA and DNA diagnostics on microspheres: current and emerging methods",
abstract = "Emerging infections and rapidly evolving pathogens are a challenge to conventional nucleic acid-based diagnostic tests. Current nucleic acid-based diagnosis is limited in the spectrum of analytes that can be simultaneously detected. This can potentially be addressed by single-tube multiplexing or multiple-well multiplexing (where multiplexing is defined as using a mixture of reagents, capable of detecting multiple diagnostic targets in a single tube or in highly parallel arrays). The number of organisms that can be targeted in a single tube by the current gold standard in clinical laboratories [quantitative real-time polymerase chain reaction (qPCR)] is limited by the number of resolvable fluorophores. The demand for multiplex technologies has led to the development of various high-throughput assays and broad-spectrum formats. In this article, current microsphere-mediated formats, their clinical applications and established microsphere-based diagnostics are reviewed. We then discuss some novel microsphere-integrated techniques and their future prospects in clinical diagnostics. We differentiate between innovations in the molecular reactions with microspheres and novel microsphere encoding strategies to increase multiplexing capacities.",
keywords = "Microsphere, PCR, Multiplex, Diagnostic, RNA, DNA, Flow cytometry",
author = "Anna Weis and Fang Liang and Jing Gao and Barnard, {Ross T.} and Simon Corrie",
note = "Publisher Copyright: {\textcopyright} 2015, Springer International Publishing Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.",
year = "2015",
doi = "10.1007/978-3-319-17305-4_10",
language = "English",
isbn = "9783319173047",
series = "RNA Technologies",
publisher = "Springer",
pages = "205--224",
editor = "Erdmann, {Volker A.} and Stefan Jurga and Jan Barciszewski",
booktitle = "RNA and DNA Diagnostics",
edition = "1",
}