RMRP is a non-coding RNA essential for early murine development

Joseph Rosenbluh, Deepak Nijhawan, Zhao Chen, Kwok-Kin Wong, Kenkichi Masutomi, William C. Hahn

Research output: Contribution to journalArticleResearchpeer-review

30 Citations (Scopus)

Abstract

RMRP is a non-coding RNA that is ubiquitously expressed in both humans and mice. RMRP mutations that lead to decreased RMRP levels are found in the pleiotropic syndrome Cartilage Hair Hypoplasia. To assess the effects of deleting RMRP, we engineered a targeting vector that contains loxP sequences flanking RMRP and created hemizygous mice harboring this engineered allele (RMRP conditional). We found that insertion of this cassette suppressed RMRP expression, and we failed to obtain viable mice homozygous for the RMRP conditional allele. Furthermore, we were unable to obtain viable homozygous RMRP null mice, indicating that RMRP is essential for early embryonic development.

Original languageEnglish
Article numbere26270
Pages (from-to)1-5
Number of pages5
JournalPLoS ONE
Volume6
Issue number10
DOIs
Publication statusPublished - Oct 2011
Externally publishedYes

Keywords

  • animal cell
  • animal experiment
  • cartilage hypoplasia
  • controlled study
  • embryo
  • embryo cell
  • embryo development
  • Female
  • gene cassette
  • gene deletion
  • gene insertion sequence
  • gene repression
  • gene sequence
  • gene targeting
  • hemizygosity
  • homozygosity
  • lethal gene
  • male
  • Mouse
  • nonhuman
  • pleiotropy
  • RMRP gene
  • RNA gene
  • allele
  • animal
  • genetics
  • molecular genetics
  • Northern blotting
  • nucleotide sequence
  • physiology
  • reverse transcription polymerase chain reaction
  • Southern blotting
  • Murinae
  • Mus
  • small interfering RNA
  • untranslated RNA
  • Base Sequence
  • Molecular Sequence Data
  • RNA, Small Interfering
  • RNA, Untranslated

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