Riquiqui and minibrain are regulators of the Hippo pathway downstream of Dachsous

Joffrey L. Degoutin, Claire C. Milton, Eefang Yu, Marla Tipping, Floris Bosveld, Liu Yang, Yohanns Bellaiche, Alexey Veraksa, Kieran F. Harvey

Research output: Contribution to journalArticleResearchpeer-review

40 Citations (Scopus)

Abstract

The atypical cadherins Fat (Ft) and Dachsous (Ds) control tissue growth through the Salvador-Warts-Hippo (SWH) pathway, and also regulate planar cell polarity and morphogenesis. Ft and Ds engage in reciprocal signalling as both proteins can serve as receptor and ligand for each other. The intracellular domains (ICDs) of Ft and Ds regulate the activity of the key SWH pathway transcriptional co-activator protein Yorkie (Yki). Signalling from the FtICD is well characterized and controls tissue growth by regulating the abundance of the Yki-repressive kinase Warts (Wts). Here we identify two regulators of the Drosophila melanogaster SWH pathway that function downstream of the DsICD: the WD40 repeat protein Riquiqui (Riq) and the DYRK-family kinase Minibrain (Mnb). Ds physically interacts with Riq, which binds to both Mnb and Wts. Riq and Mnb promote Yki-dependent tissue growth by stimulating phosphorylation-dependent inhibition of Wts. Thus, we describe a previously unknown branch of the SWH pathway that controls tissue growth downstream of Ds.

Original languageEnglish
Pages (from-to)1176-1185
Number of pages10
JournalNature Cell Biology
Volume15
Issue number10
DOIs
Publication statusPublished - 1 Oct 2013
Externally publishedYes

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