Review of microfluidic microbioreactor technology for high-throughput submerged microbiological cultivation

Hanaa M. Hegab, Ahmed ElMekawy, Tim Stakenborg

Research output: Contribution to journalArticleResearchpeer-review

46 Citations (Scopus)


Microbial fermentation process development is pursuing a high production yield. This requires a high throughput screening and optimization of the microbial strains, which is nowadays commonly achieved by applying slow and labor-intensive submerged cultivation in shake flasks or microtiter plates. These methods are also limited towards end-point measurements, low analytical data output, and control over the fermentation process. These drawbacks could be overcome by means of scaled-down microfluidic microbioreactors (μBR) that allow for online control over cultivation data and automation, hence reducing cost and time. This review goes beyond previous work not only by providing a detailed update on the current μBR fabrication techniques but also the operation and control of μBRs is compared to large scale fermentation reactors.

Original languageEnglish
Article number021502
Issue number2
Publication statusPublished - 5 Apr 2013
Externally publishedYes

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