Projects per year
Abstract
A pH dependent reversible sponge like behavior of a bovine serum albumin (BSA) nanolayer adsorbed at the gold-saline interface is revealed by quartz crystal microbalance with dissipation (QCM-D), atomic force microscope (AFM) and contact angle measurements. During the saline rinsing cycles, the BSA layer adsorbs water molecules at pH 7.0 and releases them at pH 4.5. The phenomenon remains constant and reproducible upon multiple rinsing cycles. The BSA layer thickness also increases upon rinsing with saline at pH 7.0 and reverses back to its original thickness at pH 4.5. Varying ionic strength with water further desorbs more water molecules from the BSA layer, which decreases its mass and thickness. However, upon both pH and ionic strength changes, all the BSA molecules remain adsorbed irreversibly at the gold interface and only the sorption of water molecules occurs. The study aims at engineering high efficiency pH-responsive biodiagnostics and drug delivery systems.
Original language | English |
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Article number | 573 |
Number of pages | 10 |
Journal | Frontiers in Bioengineering and Biotechnology |
Volume | 8 |
DOIs | |
Publication status | Published - 3 Jun 2020 |
Keywords
- adsorption
- AFM
- bovine serum albumin (BSA)
- contact angle
- dynamic light scattering (DLS)
- gold
- pH
- QCM-D
Projects
- 1 Finished
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ARC Research Hub for Processing Lignocellulosics into High Value Products
Garnier, G. (Primary Chief Investigator (PCI)), Batchelor, W. (Chief Investigator (CI)), Simon, G. (Chief Investigator (CI)), Haritos, V. (Chief Investigator (CI)), Patti, A. (Chief Investigator (CI)), Saito, K. (Chief Investigator (CI)), Griesser, H. (Chief Investigator (CI)), Paull, B. (Chief Investigator (CI)), Tanner, J. (Chief Investigator (CI)), Spinnler, H. (Partner Investigator (PI)), Allais, F. (Partner Investigator (PI)), Richardson, D. (Partner Investigator (PI)), Mackay, A. (Partner Investigator (PI)), Carter, S. (Partner Investigator (PI)), Faltas, R. (Partner Investigator (PI)), Edye, L. (Partner Investigator (PI)), Hendriks, D. (Partner Investigator (PI)), Karmakar, N. (Chief Investigator (CI)), Bhattacharya, S. (Chief Investigator (CI)) & Hawe, N. (Project Manager)
Monash University – Internal University Contribution, Monash University – Internal Faculty Contribution, Monash University – Internal Department Contribution, Paper Australia Pty Ltd, Leaf Resources Pty Ltd, Department of State Growth (Tasmania), University of Tasmania, University of South Australia, Agro Biotechnologies Industrielles, Visy Industries Australia Pty Ltd (trading as Visy Industries), Norske Skog Paper Mills (Australia) Pty Ltd, Orora Limited (trading as AMCOR Australia)
10/01/18 → 31/12/24
Project: Research