Reverse transcriptase mutations in sequential HIV‐1 isolates in a patient with AIDS

A. D. Gurusinghe, S. A. Land, C. Birch, C. McGavin, D. J. Hooker, G. Tachedjian, R. Doherty, N. J. Deacon

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Abstract

Sequential human immunodeficiency virus type 1 (HIV‐1) isolates were obtained over a 29‐month period from a person before, during, and after AZT therapy. DNA sequence analysis of polymerase chain‐amplified reverse‐transcriptase gene showed a gradual accumulation of mutations to peak resistance (lC50 2.13 μM AZT) in association with mutations at codons 44, 210, and 369, as well as at 41, 67, 70, and 215. Eight months after cessation of AZT therapy, when an HIV‐1 isolate from the patient was again sensitive to AZT, these mutations had all returned to the pretherapy sequence. © 1995 Wiley‐Liss, Inc.

Original languageEnglish
Pages (from-to)238-243
Number of pages6
JournalJournal of Medical Virology
Volume46
Issue number3
DOIs
Publication statusPublished - 1 Jan 1995

Keywords

  • AZT
  • HIV‐1
  • mutations
  • resistance
  • reverse
  • transcriptase

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