Retinoic acid effects on an SV-40 large T antigen immortalized adult rat bone cell line

Marie Helene Lafage-Proust, Gregg Wesolowski, Matthias Ernst, Gideon A. Rodan, Sevgi B. Rodan

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Clonal cell lines were established from adult rat tibia cells immortalized with SV-40 large T antigen. One clone (TRAB-11), in which retinoic acid (RA) induced alkaline phosphatase (AP) activity, was selected for further study. The TRAB-11 cells express high levels of type I collagen mRNA, type IV collagen, fibronectin, practically no type III collagen, little osteopontin, and no osteocalcin. RA stimulates proliferation of TRAB-11 cells (starting at 10 pM) and survival (starting at 100 pM). TRAB-11 cells synthesize fibroblast growth factor-2 (FGF-2), which has potent autocrine mitogenic effects on these cells and acts synergistically with RA. TRAB-11 cells attach better to type IV collagen than to fibronectin or laminin. Cell attachment to type IV collagen is increased by RA and decreased (65%) by an antibody directed against α1β1 integrin. RA up-regulates steady-state levels of α1 mRNA without affecting β1 mRNA expression. In conclusion, we report the establishment of a clonal cell line from the outgrowth of adult rat tibiae which is highly sensitive to RA in its growth and survival in culture, apparently as a result of integrin-mediated cell interaction with extracellular matrix proteins.

Original languageEnglish
Pages (from-to)267-275
Number of pages9
JournalJournal of Cellular Physiology
Issue number3
Publication statusPublished - 10 May 1999
Externally publishedYes

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