TY - JOUR
T1 - Retinoic acid effects on an SV-40 large T antigen immortalized adult rat bone cell line
AU - Lafage-Proust, Marie Helene
AU - Wesolowski, Gregg
AU - Ernst, Matthias
AU - Rodan, Gideon A.
AU - Rodan, Sevgi B.
PY - 1999/5/10
Y1 - 1999/5/10
N2 - Clonal cell lines were established from adult rat tibia cells immortalized with SV-40 large T antigen. One clone (TRAB-11), in which retinoic acid (RA) induced alkaline phosphatase (AP) activity, was selected for further study. The TRAB-11 cells express high levels of type I collagen mRNA, type IV collagen, fibronectin, practically no type III collagen, little osteopontin, and no osteocalcin. RA stimulates proliferation of TRAB-11 cells (starting at 10 pM) and survival (starting at 100 pM). TRAB-11 cells synthesize fibroblast growth factor-2 (FGF-2), which has potent autocrine mitogenic effects on these cells and acts synergistically with RA. TRAB-11 cells attach better to type IV collagen than to fibronectin or laminin. Cell attachment to type IV collagen is increased by RA and decreased (65%) by an antibody directed against α1β1 integrin. RA up-regulates steady-state levels of α1 mRNA without affecting β1 mRNA expression. In conclusion, we report the establishment of a clonal cell line from the outgrowth of adult rat tibiae which is highly sensitive to RA in its growth and survival in culture, apparently as a result of integrin-mediated cell interaction with extracellular matrix proteins.
AB - Clonal cell lines were established from adult rat tibia cells immortalized with SV-40 large T antigen. One clone (TRAB-11), in which retinoic acid (RA) induced alkaline phosphatase (AP) activity, was selected for further study. The TRAB-11 cells express high levels of type I collagen mRNA, type IV collagen, fibronectin, practically no type III collagen, little osteopontin, and no osteocalcin. RA stimulates proliferation of TRAB-11 cells (starting at 10 pM) and survival (starting at 100 pM). TRAB-11 cells synthesize fibroblast growth factor-2 (FGF-2), which has potent autocrine mitogenic effects on these cells and acts synergistically with RA. TRAB-11 cells attach better to type IV collagen than to fibronectin or laminin. Cell attachment to type IV collagen is increased by RA and decreased (65%) by an antibody directed against α1β1 integrin. RA up-regulates steady-state levels of α1 mRNA without affecting β1 mRNA expression. In conclusion, we report the establishment of a clonal cell line from the outgrowth of adult rat tibiae which is highly sensitive to RA in its growth and survival in culture, apparently as a result of integrin-mediated cell interaction with extracellular matrix proteins.
UR - http://www.scopus.com/inward/record.url?scp=0032940052&partnerID=8YFLogxK
U2 - 10.1002/(SICI)1097-4652(199906)179:3<267::AID-JCP4>3.0.CO;2-0
DO - 10.1002/(SICI)1097-4652(199906)179:3<267::AID-JCP4>3.0.CO;2-0
M3 - Article
C2 - 10228945
AN - SCOPUS:0032940052
SN - 0021-9541
VL - 179
SP - 267
EP - 275
JO - Journal of Cellular Physiology
JF - Journal of Cellular Physiology
IS - 3
ER -