Respiratory syncytial virus matrix protein associates with nucleocapsids in infected cells

Reena Ghildyal, J Mills, M Murray, Nicholas J Vardaxis, J Meanger

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Abstract

Little is known about the functions of the matrix (M) protein of respiratory syncytial virus (RSV). By analogy with other negative-strand RNA viruses, the M protein should inhibit the viral polymerase prior to packaging and facilitate virion assembly. In this study, localization of the RSV M protein in infected cells and its association with the RSV nucleocapsid complex was investigated. RSV-infected cells were shown to contain characteristic cytoplasmic inclusions. Further analysis showed that these inclusions were localization sites of the M protein as well as the N, P, L and M2-1 proteins described previously. The M protein co-purified with viral ribonucleoproteins (RNPs) from RSV-infected cells. The transcriptase activity of purified RNPs was enhanced by treatment with antibodies to the M protein in a dose-dependent manner. These data suggest that the M protein is associated with RSV nucleocapsids and, like the matrix proteins of other negative-strand RNA viruses, can inhibit virus transcription.
Original languageEnglish
Pages (from-to)753 - 757
Number of pages5
JournalJournal of General Virology
Volume83
Issue numberPt 4
Publication statusPublished - 2002

Cite this

Ghildyal, R., Mills, J., Murray, M., Vardaxis, N. J., & Meanger, J. (2002). Respiratory syncytial virus matrix protein associates with nucleocapsids in infected cells. Journal of General Virology, 83(Pt 4), 753 - 757.