Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production

MengJie Hu, Keith E Schulze, Reena Ghildyal, Darren C Henstridge, Jacek L. Kolanowski, Elizabeth J. New, Yuning Hong, Alan C. Hsu, Philip M. Hansbro, Peter Ab Wark, Marie A. Bogoyevitch, David A Jans

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Although respiratory syncytial virus (RSV) is responsible for more human deaths each year than influenza, its pathogenic mechanisms are poorly understood. Here high-resolution quantitative imaging, bioenergetics measurements and mitochondrial membrane potential- and redox-sensitive dyes are used to define RSV's impact on host mitochondria for the first time, delineating RSV-induced microtubule/dynein-dependent mitochondrial perinuclear clustering, and translocation towards the microtubule-organizing centre. These changes are concomitant with impaired mitochondrial respiration, loss of mitochondrial membrane potential and increased production of mitochondrial reactive oxygen species (ROS). Strikingly, agents that target microtubule integrity the dynein motor protein, or inhibit mitochondrial ROS production strongly suppresses RSV virus production, including in a mouse model with concomitantly reduced virus-induced lung inflammation. The results establish RSV's unique ability to co-opt host cell mitochondria to facilitate viral infection, revealing the RSV-mitochondrial interface for the first time as a viable target for therapeutic intervention.

Original languageEnglish
Article numbere42448
Number of pages30
JournaleLife
Volume8
DOIs
Publication statusPublished - 27 Jun 2019

Keywords

  • human
  • infection
  • infectious disease
  • microbiology
  • mitochondria
  • reactive oxygen species
  • respiratory syncytial virus

Cite this

Hu, MengJie ; Schulze, Keith E ; Ghildyal, Reena ; Henstridge, Darren C ; Kolanowski, Jacek L. ; New, Elizabeth J. ; Hong, Yuning ; Hsu, Alan C. ; Hansbro, Philip M. ; Wark, Peter Ab ; Bogoyevitch, Marie A. ; Jans, David A. / Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production. In: eLife. 2019 ; Vol. 8.
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title = "Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production",
abstract = "Although respiratory syncytial virus (RSV) is responsible for more human deaths each year than influenza, its pathogenic mechanisms are poorly understood. Here high-resolution quantitative imaging, bioenergetics measurements and mitochondrial membrane potential- and redox-sensitive dyes are used to define RSV's impact on host mitochondria for the first time, delineating RSV-induced microtubule/dynein-dependent mitochondrial perinuclear clustering, and translocation towards the microtubule-organizing centre. These changes are concomitant with impaired mitochondrial respiration, loss of mitochondrial membrane potential and increased production of mitochondrial reactive oxygen species (ROS). Strikingly, agents that target microtubule integrity the dynein motor protein, or inhibit mitochondrial ROS production strongly suppresses RSV virus production, including in a mouse model with concomitantly reduced virus-induced lung inflammation. The results establish RSV's unique ability to co-opt host cell mitochondria to facilitate viral infection, revealing the RSV-mitochondrial interface for the first time as a viable target for therapeutic intervention.",
keywords = "human, infection, infectious disease, microbiology, mitochondria, reactive oxygen species, respiratory syncytial virus",
author = "MengJie Hu and Schulze, {Keith E} and Reena Ghildyal and Henstridge, {Darren C} and Kolanowski, {Jacek L.} and New, {Elizabeth J.} and Yuning Hong and Hsu, {Alan C.} and Hansbro, {Philip M.} and Wark, {Peter Ab} and Bogoyevitch, {Marie A.} and Jans, {David A}",
year = "2019",
month = "6",
day = "27",
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Hu, M, Schulze, KE, Ghildyal, R, Henstridge, DC, Kolanowski, JL, New, EJ, Hong, Y, Hsu, AC, Hansbro, PM, Wark, PA, Bogoyevitch, MA & Jans, DA 2019, 'Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production', eLife, vol. 8, e42448. https://doi.org/10.7554/eLife.42448

Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production. / Hu, MengJie; Schulze, Keith E; Ghildyal, Reena; Henstridge, Darren C; Kolanowski, Jacek L.; New, Elizabeth J.; Hong, Yuning; Hsu, Alan C.; Hansbro, Philip M.; Wark, Peter Ab; Bogoyevitch, Marie A.; Jans, David A.

In: eLife, Vol. 8, e42448, 27.06.2019.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production

AU - Hu, MengJie

AU - Schulze, Keith E

AU - Ghildyal, Reena

AU - Henstridge, Darren C

AU - Kolanowski, Jacek L.

AU - New, Elizabeth J.

AU - Hong, Yuning

AU - Hsu, Alan C.

AU - Hansbro, Philip M.

AU - Wark, Peter Ab

AU - Bogoyevitch, Marie A.

AU - Jans, David A

PY - 2019/6/27

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N2 - Although respiratory syncytial virus (RSV) is responsible for more human deaths each year than influenza, its pathogenic mechanisms are poorly understood. Here high-resolution quantitative imaging, bioenergetics measurements and mitochondrial membrane potential- and redox-sensitive dyes are used to define RSV's impact on host mitochondria for the first time, delineating RSV-induced microtubule/dynein-dependent mitochondrial perinuclear clustering, and translocation towards the microtubule-organizing centre. These changes are concomitant with impaired mitochondrial respiration, loss of mitochondrial membrane potential and increased production of mitochondrial reactive oxygen species (ROS). Strikingly, agents that target microtubule integrity the dynein motor protein, or inhibit mitochondrial ROS production strongly suppresses RSV virus production, including in a mouse model with concomitantly reduced virus-induced lung inflammation. The results establish RSV's unique ability to co-opt host cell mitochondria to facilitate viral infection, revealing the RSV-mitochondrial interface for the first time as a viable target for therapeutic intervention.

AB - Although respiratory syncytial virus (RSV) is responsible for more human deaths each year than influenza, its pathogenic mechanisms are poorly understood. Here high-resolution quantitative imaging, bioenergetics measurements and mitochondrial membrane potential- and redox-sensitive dyes are used to define RSV's impact on host mitochondria for the first time, delineating RSV-induced microtubule/dynein-dependent mitochondrial perinuclear clustering, and translocation towards the microtubule-organizing centre. These changes are concomitant with impaired mitochondrial respiration, loss of mitochondrial membrane potential and increased production of mitochondrial reactive oxygen species (ROS). Strikingly, agents that target microtubule integrity the dynein motor protein, or inhibit mitochondrial ROS production strongly suppresses RSV virus production, including in a mouse model with concomitantly reduced virus-induced lung inflammation. The results establish RSV's unique ability to co-opt host cell mitochondria to facilitate viral infection, revealing the RSV-mitochondrial interface for the first time as a viable target for therapeutic intervention.

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