TY - JOUR
T1 - Reprogramming of somatic cells after fusion with induced pluripotent stem cells and nuclear transfer embryonic stem cells
AU - Sumer, Huseyin
AU - Jones, Karen Louise
AU - Liu, Jun
AU - Heffernan, Corey
AU - Tat, Pollyanna Agnes
AU - Upton, Kyle Ross
AU - Verma, Paul John
PY - 2010
Y1 - 2010
N2 - In this study we examine whether a somatic cell, once returned to a pluripotent state, gains the ability to reprogram
other somatic cells. We reprogrammed mouse embryonic fi broblasts by viral induction of oct4, sox2, c-myc,
and klf-4 genes. Upon fusion of the resulting iPS cells with somatic cells harboring an Oct4-GFP transgene we
observed, GFP expression along with activation of Oct4 from the somatic genome, expression of key pluripotency
genes, and positive immunostaining for Oct4, SSEA-1, and alkaline phosphatase. The iPS-somatic hybrids
had the ability to differentiate into cell types indicative of the three germ layers and were able to localize to the
inner cell mass of aggregated embryos. Furthermore, ntES cells were used as fusion partners to generate hybrids,
which were also confi rmed to be reprogrammed to a pluripotent state. These results demonstrate that once a
somatic cell nucleus is reprogrammed, it acquires the capacity and potency to reprogram other somatic cells by
cell fusion and shares this functional property with normal embryonic stem (ES) cells.
AB - In this study we examine whether a somatic cell, once returned to a pluripotent state, gains the ability to reprogram
other somatic cells. We reprogrammed mouse embryonic fi broblasts by viral induction of oct4, sox2, c-myc,
and klf-4 genes. Upon fusion of the resulting iPS cells with somatic cells harboring an Oct4-GFP transgene we
observed, GFP expression along with activation of Oct4 from the somatic genome, expression of key pluripotency
genes, and positive immunostaining for Oct4, SSEA-1, and alkaline phosphatase. The iPS-somatic hybrids
had the ability to differentiate into cell types indicative of the three germ layers and were able to localize to the
inner cell mass of aggregated embryos. Furthermore, ntES cells were used as fusion partners to generate hybrids,
which were also confi rmed to be reprogrammed to a pluripotent state. These results demonstrate that once a
somatic cell nucleus is reprogrammed, it acquires the capacity and potency to reprogram other somatic cells by
cell fusion and shares this functional property with normal embryonic stem (ES) cells.
UR - http://www.liebertonline.com/doi/pdf/10.1089/scd.2009.0142
U2 - 10.1089/scd.2009.0142
DO - 10.1089/scd.2009.0142
M3 - Article
VL - 19
SP - 239
EP - 246
JO - Stem Cells and Development
JF - Stem Cells and Development
SN - 1547-3287
IS - 2
ER -