Reprogramming of somatic cells after fusion with induced pluripotent stem cells and nuclear transfer embryonic stem cells

Huseyin Sumer, Karen Louise Jones, Jun Liu, Corey Heffernan, Pollyanna Agnes Tat, Kyle Ross Upton, Paul John Verma

    Research output: Contribution to journalArticleResearchpeer-review

    22 Citations (Scopus)

    Abstract

    In this study we examine whether a somatic cell, once returned to a pluripotent state, gains the ability to reprogram other somatic cells. We reprogrammed mouse embryonic fi broblasts by viral induction of oct4, sox2, c-myc, and klf-4 genes. Upon fusion of the resulting iPS cells with somatic cells harboring an Oct4-GFP transgene we observed, GFP expression along with activation of Oct4 from the somatic genome, expression of key pluripotency genes, and positive immunostaining for Oct4, SSEA-1, and alkaline phosphatase. The iPS-somatic hybrids had the ability to differentiate into cell types indicative of the three germ layers and were able to localize to the inner cell mass of aggregated embryos. Furthermore, ntES cells were used as fusion partners to generate hybrids, which were also confi rmed to be reprogrammed to a pluripotent state. These results demonstrate that once a somatic cell nucleus is reprogrammed, it acquires the capacity and potency to reprogram other somatic cells by cell fusion and shares this functional property with normal embryonic stem (ES) cells.
    Original languageEnglish
    Pages (from-to)239 - 246
    Number of pages22
    JournalStem Cells and Development
    Volume19
    Issue number2
    DOIs
    Publication statusPublished - 2010

    Cite this