Reprogramming of mouse amniotic fluid cells using a PiggyBac transposon system

E. Bertin, M. Piccoli, C. Franzin, A. Nagy, M. Mileikovsky, P. De Coppi, M. Pozzobon

Research output: Contribution to journalEditorialResearchpeer-review

6 Citations (Scopus)

Abstract

Induced pluripotent stem (iPS) cells are generated from mouse and human somatic cells by forced expression of defined transcription factors using different methods. Amniotic fluid (AF) cells are easy to obtain from routinely scheduled procedures for prenatal diagnosis and iPS cells have been generated from human AF. Here, we generated iPS cells from mouse AF cells, using a non-viral-based approach constituted by the PiggyBac (PB) transposon system. All iPS cell lines obtained exhibited characteristics of pluripotent cells, including the ability to differentiate toward derivatives of all three germ layers in vitro and in vivo.

Original languageEnglish
Pages (from-to)510-513
Number of pages4
JournalStem Cell Research
Volume15
Issue number3
DOIs
Publication statusPublished - 1 Nov 2015
Externally publishedYes

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