Reprogramming mouse fibroblasts with piggyBac transposons

Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy, Andras Nagy

Research output: Contribution to journalArticleOtherpeer-review

Abstract

In 2006, Shinya Yamanaka and his student Kazutoshi Takahashi showed that the expression of only four specific genes is sufficient to reprogram fully differentiated somatic cells into pluripotent stem cells. These cells, termed induced pluripotent stem (iPS) cells, share many of their characteristics with embryonic stem (ES) cells. In this protocol, we describe one of the simplest ways of generating iPS cells from mouse fibroblasts. It combines an efficient transposon-mediated transfection and the tetracy-cline-inducible system to control the expression of the Yamanaka reprogramming factors.

Original languageEnglish
Pages (from-to)827-830
Number of pages4
JournalCold Spring Harbor Protocols
Volume2017
Issue number10
DOIs
Publication statusPublished - 1 Oct 2017

Cite this

Behringer, Richard ; Gertsenstein, Marina ; Vintersten Nagy, Kristina ; Nagy, Andras. / Reprogramming mouse fibroblasts with piggyBac transposons. In: Cold Spring Harbor Protocols. 2017 ; Vol. 2017, No. 10. pp. 827-830.
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Behringer, R, Gertsenstein, M, Vintersten Nagy, K & Nagy, A 2017, 'Reprogramming mouse fibroblasts with piggyBac transposons' Cold Spring Harbor Protocols, vol. 2017, no. 10, pp. 827-830. https://doi.org/10.1101/pdb.prot092627

Reprogramming mouse fibroblasts with piggyBac transposons. / Behringer, Richard; Gertsenstein, Marina; Vintersten Nagy, Kristina; Nagy, Andras.

In: Cold Spring Harbor Protocols, Vol. 2017, No. 10, 01.10.2017, p. 827-830.

Research output: Contribution to journalArticleOtherpeer-review

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