Replica moulded poly(dimethylsiloxane) microwell arrays induce localized endothelial cell immobilization for coculture with pancreatic islets

Anouck L.S. Burzava, Aurelien Forget, Frances J. Harding, Michaelia P. Cockshell, Daniella Penko, Camille Rouzaud, Vincent Ahmadi, Paula F. Marina, Darling Rojas-Canales, Claudine S. Bonder, P. Toby H. Coates, Michaela Waibel, Helen E. Thomas, Thomas W. Kay, Thomas Loudovaris, Anton Blencowe, Nicolas H. Voelcker

Research output: Contribution to journalArticleResearchpeer-review

Abstract

PolyJet three-dimensional (3D) printing allows for the rapid manufacturing of 3D moulds for the fabrication of cross-linked poly(dimethylsiloxane) microwell arrays (PMAs). As this 3D printing technique has a resolution on the micrometer scale, the moulds exhibit a distinct surface roughness. In this study, the authors demonstrate by optical profilometry that the topography of the 3D printed moulds can be transferred to the PMAs and that this roughness induced cell adhesive properties to the material. In particular, the topography facilitated immobilization of endothelial cells on the internal walls of the microwells. The authors also demonstrate that upon immobilization of endothelial cells to the microwells, a second population of cells, namely, pancreatic islets could be introduced, thus producing a 3D coculture platform.

Original languageEnglish
Article number011002
Number of pages8
JournalBiointerphases
Volume14
Issue number1
DOIs
Publication statusPublished - 30 Jan 2019

Cite this