Repetitive sperm-induced Ca2+ transients in mouse oocytes are cell cycle dependent

K. T. Jones, J. Carroll, J. A. Merriman, D. G. Whittingham, T. Kono

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152 Citations (Scopus)

Abstract

Mature mouse oocytes are arrested at metaphase of the second meiotic division. Completion of meiosis and a block to polyspermy is caused by a series of repetitive Ca2+ transients triggered by the sperm at fertilization. These Ca2+ transients have been widely reported to last for a number of hours but when, or why, they cease is not known. Here we show that Ca2+ transients cease during entry into interphase, at the time when pronuclei are forming. In fertilized oocytes arrested at metaphase using colcemid, Ca2+ transients continued for as long as measurements were made, up to 18 hours after fertilization. Therefore sperm is able to induce Ca2+ transients during metaphase but not during interphase. In addition metaphase II oocytes, but not pronuclear stage 1-cell embryos showed highly repetitive Ca2+ oscillations in response to microinjection of inositol trisphosphate. This was explored further by treating in vitro maturing oocytes at metaphase I for 4-5 hours with cycloheximide, which induced nuclear progression to interphase (nucleus formation) and subsequent re-entry to metaphase (nuclear envelope breakdown). Fertilization of cycloheximide-treated oocytes revealed that continuous Ca2+ oscillations in response to sperm were observed after nuclear envelope breakdown but not during interphase. However interphase oocytes were able to generate Ca2+ transients in response to thimerosal. This data suggests that the ability of the sperm to trigger repetitive Ca2+ transients in oocytes is modulated in a cell cycle-dependent manner.

Original languageEnglish
Pages (from-to)3259-3266
Number of pages8
JournalDevelopment
Volume121
Issue number10
Publication statusPublished - 1 Jan 1995
Externally publishedYes

Keywords

  • Ca transients
  • Cycloheximide
  • Fertilization
  • Meiosis
  • Mitosis
  • Mouse oocyte

Cite this

Jones, K. T., Carroll, J., Merriman, J. A., Whittingham, D. G., & Kono, T. (1995). Repetitive sperm-induced Ca2+ transients in mouse oocytes are cell cycle dependent. Development, 121(10), 3259-3266.
Jones, K. T. ; Carroll, J. ; Merriman, J. A. ; Whittingham, D. G. ; Kono, T. / Repetitive sperm-induced Ca2+ transients in mouse oocytes are cell cycle dependent. In: Development. 1995 ; Vol. 121, No. 10. pp. 3259-3266.
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Jones, KT, Carroll, J, Merriman, JA, Whittingham, DG & Kono, T 1995, 'Repetitive sperm-induced Ca2+ transients in mouse oocytes are cell cycle dependent', Development, vol. 121, no. 10, pp. 3259-3266.

Repetitive sperm-induced Ca2+ transients in mouse oocytes are cell cycle dependent. / Jones, K. T.; Carroll, J.; Merriman, J. A.; Whittingham, D. G.; Kono, T.

In: Development, Vol. 121, No. 10, 01.01.1995, p. 3259-3266.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Carroll, J.

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Jones KT, Carroll J, Merriman JA, Whittingham DG, Kono T. Repetitive sperm-induced Ca2+ transients in mouse oocytes are cell cycle dependent. Development. 1995 Jan 1;121(10):3259-3266.