Reperfusion-induced myocardial dysfunction is prevented by endogenous annexin-A1 and its N-terminal-derived peptide Ac-ANX-A1{2-26}

Chengxue Qin, Keith David Buxton, Salvatore Pepe, Anh Cao, Kylie M Venardos, Jane E Love, David M Kaye, Yuan Hang Yang, Eric Francis Morand, Rebecca H Ritchie

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39 Citations (Scopus)

Abstract

Annexin-A1(ANX-A1) is an endogenous, glucocorticoid-regulated anti-inflammatory protein. The N-terminal derived peptide Ac-ANX-A1 (2-26) preserves cardiomyocyte viability, but the impact of ANX-A1-peptides on cardiac contractility is unknown. We now test the hypothesis that ANX-A1 preserves post-ischaemic recovery of left ventricular (LV) function. EXPERIMENTAL APPROACH: Ac-ANX-A1 (2-26) was administered on reperfusion, to adult rat cardiomyocytes as well as hearts isolated from rats, wild-type mice and mice deficient in endogenous ANX-A1 (ANX-A1 (-/-) ). Myocardial viability and recovery of LV function were determined. KEY RESULTS: Ischaemia-reperfusion markedly impaired both cardiomyocyte viability, and recovery of LV function by 60 . Treatment with exogenous Ac-ANX-A1 (2-26) at the onset of reperfusion prevented cardiomyocyte injury and significantly improved recovery of LV function, in both intact rat and wild-type mouse hearts. Ac-ANX-A1 (2-26) cardioprotection was abolished by either formyl peptide receptor (FPR)-nonselective or FPR1-selective antagonists, Boc2 and cyclosporin H, but was relatively insensitive to the FPR2-selective antagonist QuinC7. ANX-A1-induced cardioprotection was associated with increased phosphorylation of the cell survival kinase Akt. ANX-A1 (-/-) exaggerated impairment of post-ischaemic recovery of LV function, in addition to selective LV FPR1 downregulation. CONCLUSIONS AND IMPLICATIONS: These data represent the first evidence that ANX-A1 impacts on myocardial function. Our findings suggest ANX-A1 is an endogenous regulator of post-ischaemic recovery of LV function. Furthermore, the ANX-A1-derived peptide Ac-ANX-A1 (2-26) on reperfusion rescues LV function, likely via activation of FPR1. ANX-A1-based therapies may thus represent a novel clinical approach for the prevention and treatment of myocardial reperfusion injury.
Original languageEnglish
Pages (from-to)238 - 252
Number of pages15
JournalBritish Journal of Pharmacology
Volume168
Issue number1
DOIs
Publication statusPublished - 2013

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