Repeated ethanol exposure during late gestation alters the maturation and innate immune status of the ovine fetal lung

Foula Sozo, Luke Daniel O'Day, Gert Maritz, Kelly Rebecca Kenna, Victoria Stacy, Nadine Brew, David William Walker, Alan D Bocking, James F Brien, Richard Harding

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Abstract

Little is known about the effects of fetal ethanol exposure on lung development. Our aim was to determine the effects of repeated ethanol exposure during late gestation on fetal lung growth, maturation and inflammatory status. Pregnant ewes were chronically catheterized at 91 days of gestational age (DGA; term 147d). From 95-133 DGA, ewes were given a one hour daily infusion of either 0.75 g ethanol/kg (n=9) or saline (n=8), with tissue collection at 134 DGA. Fetal lungs were examined for changes in tissue growth, structure, maturation, inflammation and oxidative stress. Between treatment groups there were no differences in lung weight, DNA and protein contents, percent proliferating and apoptotic cells, tissue and air-space fractions, alveolar number and mean linear intercept, septal thickness, type-II cell number and elastin content. Ethanol exposure caused a 75 increase in pulmonary collagen I alpha1 mRNA levels (p
Original languageEnglish
Pages (from-to)1 - 38
Number of pages38
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Volume296
Publication statusPublished - 2009

Cite this

Sozo, F., O'Day, L. D., Maritz, G., Kenna, K. R., Stacy, V., Brew, N., Walker, D. W., Bocking, A. D., Brien, J. F., & Harding, R. (2009). Repeated ethanol exposure during late gestation alters the maturation and innate immune status of the ovine fetal lung. American Journal of Physiology - Lung Cellular and Molecular Physiology, 296, 1 - 38.