Renin-containing Muller cells of the retina display endocrine features

J. L. Berka, A. J. Stubbs, D. Z M Wang, R. DiNicolantonio, D. Alcorn, D. J. Campbell, S. L. Skinner

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Abstract

Purpose. An ocular renin-angiotensin system has been implicated in the proliferation of retinal blood vessels and blindness in diabetes mellitus. Its cellular basis has not been established. The objective was to identify sites of renin synthesis, secretion, and processing in eyes from humans, BALB/c mice, Sprague-Dawley rats, and a hypertensive transgenic rat model (mREN-2) that displays amplified extrarenal renin synthesis. Methods. Paraffin sections of eyes were incubated with antisera to renin protein, prorenin, vimentin, and Muller cells. Enzyme kinetic renin assay was performed on extracts of whole eyes (excluding lens and vitreous) and comparisons made with adrenal glands and kidneys. For detection of renin mRNA, retinas were separately pooled from BALB/c and Swiss mice. Results. In normal rodent and autopsy human eyes, labeling for renin, vimentin, and Muller cell protein was observed in the cytoplasm of all macroglial Muller cells, with renin labeling most obvious in endfeet closely apposed to retinal blood vessels. Prorenin labeling was not detected. Less intense renin labeling, again without prorenin, was seen in nonpigmented ciliary epithelium of rodents. In transgenic (mREN-2) rat eyes, renin and prorenin labeling of Muller cells and nonpigmented ciliary epithelium were intense. Prorenin was localized to the posterior region of Muller cells but only sparsely to endfeet in rodent retinas, and renin was present only in an active form in amounts one third that of one adrenal. Renin mRNA was readily detected. In human retina, renin was present in active and pro-forms, and the total amount was approximately one fiftieth that of adrenal. Conclusion. Renin is synthesized in the retina and is specifically localized to the macroglial Muller cells. Nonpigmented ciliary epithelium also contains renin. The presence of prorenin in the posterior part of the Muller cell, with active renin throughout but notably in endfeet in apposition to retinal capillaries, suggests directional processing of renin. These findings are consistent with earlier suggestions that retinal neovascularization may be associated with Muller cell dysfunction.

Original languageEnglish
Pages (from-to)1450-1458
Number of pages9
JournalInvestigative Ophthalmology and Visual Science
Volume36
Issue number7
Publication statusPublished - 1995
Externally publishedYes

Keywords

  • angiotensin
  • Muller cells
  • proliferative diabetic retinopathy
  • renin
  • retina

Cite this

Berka, J. L., Stubbs, A. J., Wang, D. Z. M., DiNicolantonio, R., Alcorn, D., Campbell, D. J., & Skinner, S. L. (1995). Renin-containing Muller cells of the retina display endocrine features. Investigative Ophthalmology and Visual Science, 36(7), 1450-1458.
Berka, J. L. ; Stubbs, A. J. ; Wang, D. Z M ; DiNicolantonio, R. ; Alcorn, D. ; Campbell, D. J. ; Skinner, S. L. / Renin-containing Muller cells of the retina display endocrine features. In: Investigative Ophthalmology and Visual Science. 1995 ; Vol. 36, No. 7. pp. 1450-1458.
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title = "Renin-containing Muller cells of the retina display endocrine features",
abstract = "Purpose. An ocular renin-angiotensin system has been implicated in the proliferation of retinal blood vessels and blindness in diabetes mellitus. Its cellular basis has not been established. The objective was to identify sites of renin synthesis, secretion, and processing in eyes from humans, BALB/c mice, Sprague-Dawley rats, and a hypertensive transgenic rat model (mREN-2) that displays amplified extrarenal renin synthesis. Methods. Paraffin sections of eyes were incubated with antisera to renin protein, prorenin, vimentin, and Muller cells. Enzyme kinetic renin assay was performed on extracts of whole eyes (excluding lens and vitreous) and comparisons made with adrenal glands and kidneys. For detection of renin mRNA, retinas were separately pooled from BALB/c and Swiss mice. Results. In normal rodent and autopsy human eyes, labeling for renin, vimentin, and Muller cell protein was observed in the cytoplasm of all macroglial Muller cells, with renin labeling most obvious in endfeet closely apposed to retinal blood vessels. Prorenin labeling was not detected. Less intense renin labeling, again without prorenin, was seen in nonpigmented ciliary epithelium of rodents. In transgenic (mREN-2) rat eyes, renin and prorenin labeling of Muller cells and nonpigmented ciliary epithelium were intense. Prorenin was localized to the posterior region of Muller cells but only sparsely to endfeet in rodent retinas, and renin was present only in an active form in amounts one third that of one adrenal. Renin mRNA was readily detected. In human retina, renin was present in active and pro-forms, and the total amount was approximately one fiftieth that of adrenal. Conclusion. Renin is synthesized in the retina and is specifically localized to the macroglial Muller cells. Nonpigmented ciliary epithelium also contains renin. The presence of prorenin in the posterior part of the Muller cell, with active renin throughout but notably in endfeet in apposition to retinal capillaries, suggests directional processing of renin. These findings are consistent with earlier suggestions that retinal neovascularization may be associated with Muller cell dysfunction.",
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Berka, JL, Stubbs, AJ, Wang, DZM, DiNicolantonio, R, Alcorn, D, Campbell, DJ & Skinner, SL 1995, 'Renin-containing Muller cells of the retina display endocrine features', Investigative Ophthalmology and Visual Science, vol. 36, no. 7, pp. 1450-1458.

Renin-containing Muller cells of the retina display endocrine features. / Berka, J. L.; Stubbs, A. J.; Wang, D. Z M; DiNicolantonio, R.; Alcorn, D.; Campbell, D. J.; Skinner, S. L.

In: Investigative Ophthalmology and Visual Science, Vol. 36, No. 7, 1995, p. 1450-1458.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Renin-containing Muller cells of the retina display endocrine features

AU - Berka, J. L.

AU - Stubbs, A. J.

AU - Wang, D. Z M

AU - DiNicolantonio, R.

AU - Alcorn, D.

AU - Campbell, D. J.

AU - Skinner, S. L.

PY - 1995

Y1 - 1995

N2 - Purpose. An ocular renin-angiotensin system has been implicated in the proliferation of retinal blood vessels and blindness in diabetes mellitus. Its cellular basis has not been established. The objective was to identify sites of renin synthesis, secretion, and processing in eyes from humans, BALB/c mice, Sprague-Dawley rats, and a hypertensive transgenic rat model (mREN-2) that displays amplified extrarenal renin synthesis. Methods. Paraffin sections of eyes were incubated with antisera to renin protein, prorenin, vimentin, and Muller cells. Enzyme kinetic renin assay was performed on extracts of whole eyes (excluding lens and vitreous) and comparisons made with adrenal glands and kidneys. For detection of renin mRNA, retinas were separately pooled from BALB/c and Swiss mice. Results. In normal rodent and autopsy human eyes, labeling for renin, vimentin, and Muller cell protein was observed in the cytoplasm of all macroglial Muller cells, with renin labeling most obvious in endfeet closely apposed to retinal blood vessels. Prorenin labeling was not detected. Less intense renin labeling, again without prorenin, was seen in nonpigmented ciliary epithelium of rodents. In transgenic (mREN-2) rat eyes, renin and prorenin labeling of Muller cells and nonpigmented ciliary epithelium were intense. Prorenin was localized to the posterior region of Muller cells but only sparsely to endfeet in rodent retinas, and renin was present only in an active form in amounts one third that of one adrenal. Renin mRNA was readily detected. In human retina, renin was present in active and pro-forms, and the total amount was approximately one fiftieth that of adrenal. Conclusion. Renin is synthesized in the retina and is specifically localized to the macroglial Muller cells. Nonpigmented ciliary epithelium also contains renin. The presence of prorenin in the posterior part of the Muller cell, with active renin throughout but notably in endfeet in apposition to retinal capillaries, suggests directional processing of renin. These findings are consistent with earlier suggestions that retinal neovascularization may be associated with Muller cell dysfunction.

AB - Purpose. An ocular renin-angiotensin system has been implicated in the proliferation of retinal blood vessels and blindness in diabetes mellitus. Its cellular basis has not been established. The objective was to identify sites of renin synthesis, secretion, and processing in eyes from humans, BALB/c mice, Sprague-Dawley rats, and a hypertensive transgenic rat model (mREN-2) that displays amplified extrarenal renin synthesis. Methods. Paraffin sections of eyes were incubated with antisera to renin protein, prorenin, vimentin, and Muller cells. Enzyme kinetic renin assay was performed on extracts of whole eyes (excluding lens and vitreous) and comparisons made with adrenal glands and kidneys. For detection of renin mRNA, retinas were separately pooled from BALB/c and Swiss mice. Results. In normal rodent and autopsy human eyes, labeling for renin, vimentin, and Muller cell protein was observed in the cytoplasm of all macroglial Muller cells, with renin labeling most obvious in endfeet closely apposed to retinal blood vessels. Prorenin labeling was not detected. Less intense renin labeling, again without prorenin, was seen in nonpigmented ciliary epithelium of rodents. In transgenic (mREN-2) rat eyes, renin and prorenin labeling of Muller cells and nonpigmented ciliary epithelium were intense. Prorenin was localized to the posterior region of Muller cells but only sparsely to endfeet in rodent retinas, and renin was present only in an active form in amounts one third that of one adrenal. Renin mRNA was readily detected. In human retina, renin was present in active and pro-forms, and the total amount was approximately one fiftieth that of adrenal. Conclusion. Renin is synthesized in the retina and is specifically localized to the macroglial Muller cells. Nonpigmented ciliary epithelium also contains renin. The presence of prorenin in the posterior part of the Muller cell, with active renin throughout but notably in endfeet in apposition to retinal capillaries, suggests directional processing of renin. These findings are consistent with earlier suggestions that retinal neovascularization may be associated with Muller cell dysfunction.

KW - angiotensin

KW - Muller cells

KW - proliferative diabetic retinopathy

KW - renin

KW - retina

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Berka JL, Stubbs AJ, Wang DZM, DiNicolantonio R, Alcorn D, Campbell DJ et al. Renin-containing Muller cells of the retina display endocrine features. Investigative Ophthalmology and Visual Science. 1995;36(7):1450-1458.