Removal of interference in the differential pulse polarographic determination of progestogens in some combined low-dosage oral contraceptives

Previously published differential pulse methods for the determination of certain progestogens (laevonorgestrel and norethisterone) are not applicable to combined low-dosage oral contraceptives because interference from excipients in the tablets completely eliminates the polarographic response. An ultrafiltration device allows rapid prior extraction of the interfering substances before the polarographic determination in 50% ( v v) methanol-phosphate buffer (pH 6.0). Recoveries of 100 ± 1% were obtained by the recommended method, and data for a range of formulations are in excellent agreement with expected values. Electrode characteristics of the progestogens and interfering substances are reported, based on studies employing cyclic voltammetry at a hanging mercury drop electrode, a.c. polarography and normal pulse polarography. Competitive adsorption processes seem to occur when the progestogen and excipients are simultaneously present.