TY - JOUR
T1 - Reliability, robustness, and reproducibility in mouse behavioral phenotyping: A cross-laboratory study
AU - Mandillo, Silvia
AU - Tucci, Valter
AU - Holter, Sabine M
AU - Meziane, Hamid
AU - Al Banchaabouchi, Mumna
AU - Kallnik, Magdalena
AU - Lad, Heena V
AU - Nolan, Patrick M
AU - Ouagazzal, Abdel-Mouttalib
AU - Coghill, Emma L
AU - Gale, Karin
AU - Golini, Elisabetta
AU - Jacquot, Sylvie
AU - Krezel, Wojtek
AU - Parker, Andy
AU - Riet, Fabrice
AU - Schneider, Ilka
AU - Marazziti, Daniela
AU - Auwerx, Johan
AU - Brown, Steve D M
AU - Chambon, Pierre
AU - Rosenthal, Nadia Alicia
AU - Tocchini-Valentini, Glauco
AU - Wurst, Wolfgang
PY - 2008
Y1 - 2008
N2 - Establishing standard operating procedures (SOPs) as tools for the analysis of behavioural phenotypes is fundamental to mouse functional genomics. It is essential that the tests designed provide reliable measures of the process under investigation but most importantly that these are reproducible across both time and laboratories. For this reason, we devised and tested a set of SOPs to investigate mouse behaviour. Five research centres were involved across France, Germany, Italy and the UK in this study, as part of the EUMORPHIA program. All the procedures underwent a cross-validation experimental study to investigate the robustness of the designed protocols. Four inbred reference strains (C57BL/6J, C3HeB/FeJ, BALB/cByJ, 129S2/SvPas), reflecting their use as common background strains in mutagenesis programmes, were analysed to validate these tests. We demonstrate that the operating procedures employed, which includes open field, SHIRPA, grip-strength, rotarod, Y-maze, pre-pulse inhibition and tail flick tests, generated reproducible results between laboratories for a number of the test output parameters. However, we also identified several uncontrolled variables that constitute confounding factors in behavioral phenotyping. The EUMORPHIA SOPs described here are an important start-point for the ongoing development of increasingly robust phenotyping platforms and their application in large-scale, multi-centre mouse phenotyping programmes. Key words: inbred mouse strains, behavioral phenotyping, test battery.
AB - Establishing standard operating procedures (SOPs) as tools for the analysis of behavioural phenotypes is fundamental to mouse functional genomics. It is essential that the tests designed provide reliable measures of the process under investigation but most importantly that these are reproducible across both time and laboratories. For this reason, we devised and tested a set of SOPs to investigate mouse behaviour. Five research centres were involved across France, Germany, Italy and the UK in this study, as part of the EUMORPHIA program. All the procedures underwent a cross-validation experimental study to investigate the robustness of the designed protocols. Four inbred reference strains (C57BL/6J, C3HeB/FeJ, BALB/cByJ, 129S2/SvPas), reflecting their use as common background strains in mutagenesis programmes, were analysed to validate these tests. We demonstrate that the operating procedures employed, which includes open field, SHIRPA, grip-strength, rotarod, Y-maze, pre-pulse inhibition and tail flick tests, generated reproducible results between laboratories for a number of the test output parameters. However, we also identified several uncontrolled variables that constitute confounding factors in behavioral phenotyping. The EUMORPHIA SOPs described here are an important start-point for the ongoing development of increasingly robust phenotyping platforms and their application in large-scale, multi-centre mouse phenotyping programmes. Key words: inbred mouse strains, behavioral phenotyping, test battery.
M3 - Article
SN - 1094-8341
VL - 34
SP - 243
EP - 255
JO - Physiological Genomics
JF - Physiological Genomics
IS - 3
ER -