Relaxin reverses cardiac and renal fibrosis in spontaneously hypertensive rats

Edna Lekgabe; Helen Kiriazis; Chongxin Zhao; Qi Xu; Xiao-Lei Moore; Yidan Su; Ross Bathgate; Xiao-Jun Du; Chrishan Samuel

doi:10.1161/01.HYP.0000171930.00697.2f

Relaxin reverses cardiac and renal fibrosis in spontaneously hypertensive rats

Edna Lekgabe, Helen Kiriazis, Chongxin Zhao, Qi Xu, Xiao-Lei Moore, Yidan Su, Ross Bathgate, Xiao-Jun Du, Chrishan Samuel

Research output: Contribution to journal › Article › Research › peer-review

167 Citations (Scopus)

Abstract

The antifibrotic effects of the peptide hormone relaxin on cardiac and renal fibrosis were studied in 9- to 10-month-old male spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY). Rats (n=8 to 9 per group) were allocated into 3 groups: WKY controls, vehicle-treated SHR (SHR-V), and relaxin-treated SHR (SHR-R). Relaxin (0.5 mg/kg per day) was administered via subcutaneously implanted osmotic mini-pumps over 2 weeks before hearts and kidneys were harvested for analysis. Collagen content was analyzed by hydroxyproline assay, gel electrophoresis, and quantitative histology. Zymography was used to determine matrix metalloproteinase (MMP) expression and Western blotting to determine proliferating cell nuclear antigen (PCNA) expression and alpha-smooth muscle actin (alpha-SMA)/myofibroblast expression, whereas cardiac hypertrophy was assessed by myocyte size and real-time polymerase chain reaction of associated genes. The left ventricular (LV) myocardium of SHR-V contained increased collagen levels (by 25+/-1 , P

Original language	English
Pages (from-to)	412 - 418
Number of pages	7
Journal	Hypertension
Volume	46
Issue number	2
DOIs	https://doi.org/10.1161/01.HYP.0000171930.00697.2f
Publication status	Published - 2005
Externally published	Yes

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title = "Relaxin reverses cardiac and renal fibrosis in spontaneously hypertensive rats",

abstract = "The antifibrotic effects of the peptide hormone relaxin on cardiac and renal fibrosis were studied in 9- to 10-month-old male spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY). Rats (n=8 to 9 per group) were allocated into 3 groups: WKY controls, vehicle-treated SHR (SHR-V), and relaxin-treated SHR (SHR-R). Relaxin (0.5 mg/kg per day) was administered via subcutaneously implanted osmotic mini-pumps over 2 weeks before hearts and kidneys were harvested for analysis. Collagen content was analyzed by hydroxyproline assay, gel electrophoresis, and quantitative histology. Zymography was used to determine matrix metalloproteinase (MMP) expression and Western blotting to determine proliferating cell nuclear antigen (PCNA) expression and alpha-smooth muscle actin (alpha-SMA)/myofibroblast expression, whereas cardiac hypertrophy was assessed by myocyte size and real-time polymerase chain reaction of associated genes. The left ventricular (LV) myocardium of SHR-V contained increased collagen levels (by 25+/-1 , P",

author = "Edna Lekgabe and Helen Kiriazis and Chongxin Zhao and Qi Xu and Xiao-Lei Moore and Yidan Su and Ross Bathgate and Xiao-Jun Du and Chrishan Samuel",

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doi = "10.1161/01.HYP.0000171930.00697.2f",

language = "English",

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T1 - Relaxin reverses cardiac and renal fibrosis in spontaneously hypertensive rats

AU - Lekgabe, Edna

AU - Kiriazis, Helen

AU - Zhao, Chongxin

AU - Xu, Qi

AU - Moore, Xiao-Lei

AU - Su, Yidan

AU - Bathgate, Ross

AU - Du, Xiao-Jun

AU - Samuel, Chrishan

PY - 2005

Y1 - 2005

N2 - The antifibrotic effects of the peptide hormone relaxin on cardiac and renal fibrosis were studied in 9- to 10-month-old male spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY). Rats (n=8 to 9 per group) were allocated into 3 groups: WKY controls, vehicle-treated SHR (SHR-V), and relaxin-treated SHR (SHR-R). Relaxin (0.5 mg/kg per day) was administered via subcutaneously implanted osmotic mini-pumps over 2 weeks before hearts and kidneys were harvested for analysis. Collagen content was analyzed by hydroxyproline assay, gel electrophoresis, and quantitative histology. Zymography was used to determine matrix metalloproteinase (MMP) expression and Western blotting to determine proliferating cell nuclear antigen (PCNA) expression and alpha-smooth muscle actin (alpha-SMA)/myofibroblast expression, whereas cardiac hypertrophy was assessed by myocyte size and real-time polymerase chain reaction of associated genes. The left ventricular (LV) myocardium of SHR-V contained increased collagen levels (by 25+/-1 , P

AB - The antifibrotic effects of the peptide hormone relaxin on cardiac and renal fibrosis were studied in 9- to 10-month-old male spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY). Rats (n=8 to 9 per group) were allocated into 3 groups: WKY controls, vehicle-treated SHR (SHR-V), and relaxin-treated SHR (SHR-R). Relaxin (0.5 mg/kg per day) was administered via subcutaneously implanted osmotic mini-pumps over 2 weeks before hearts and kidneys were harvested for analysis. Collagen content was analyzed by hydroxyproline assay, gel electrophoresis, and quantitative histology. Zymography was used to determine matrix metalloproteinase (MMP) expression and Western blotting to determine proliferating cell nuclear antigen (PCNA) expression and alpha-smooth muscle actin (alpha-SMA)/myofibroblast expression, whereas cardiac hypertrophy was assessed by myocyte size and real-time polymerase chain reaction of associated genes. The left ventricular (LV) myocardium of SHR-V contained increased collagen levels (by 25+/-1 , P

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U2 - 10.1161/01.HYP.0000171930.00697.2f

DO - 10.1161/01.HYP.0000171930.00697.2f

M3 - Article

SN - 0194-911X

VL - 46

SP - 412

EP - 418

JO - Hypertension

JF - Hypertension

IS - 2

ER -

Relaxin reverses cardiac and renal fibrosis in spontaneously hypertensive rats

Abstract

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