Relaxin reverses cardiac and renal fibrosis in spontaneously hypertensive rats

Edna Lekgabe, Helen Kiriazis, Chongxin Zhao, Qi Xu, Xiao-Lei Moore, Yidan Su, Ross Bathgate, Xiao-Jun Du, Chrishan Samuel

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Abstract

The antifibrotic effects of the peptide hormone relaxin on cardiac and renal fibrosis were studied in 9- to 10-month-old male spontaneously hypertensive rats (SHR) and normotensive Wistar-Kyoto rats (WKY). Rats (n=8 to 9 per group) were allocated into 3 groups: WKY controls, vehicle-treated SHR (SHR-V), and relaxin-treated SHR (SHR-R). Relaxin (0.5 mg/kg per day) was administered via subcutaneously implanted osmotic mini-pumps over 2 weeks before hearts and kidneys were harvested for analysis. Collagen content was analyzed by hydroxyproline assay, gel electrophoresis, and quantitative histology. Zymography was used to determine matrix metalloproteinase (MMP) expression and Western blotting to determine proliferating cell nuclear antigen (PCNA) expression and alpha-smooth muscle actin (alpha-SMA)/myofibroblast expression, whereas cardiac hypertrophy was assessed by myocyte size and real-time polymerase chain reaction of associated genes. The left ventricular (LV) myocardium of SHR-V contained increased collagen levels (by 25+/-1 , P
Original languageEnglish
Pages (from-to)412 - 418
Number of pages7
JournalHypertension
Volume46
Issue number2
DOIs
Publication statusPublished - 2005
Externally publishedYes

Cite this

Lekgabe, E., Kiriazis, H., Zhao, C., Xu, Q., Moore, X-L., Su, Y., Bathgate, R., Du, X-J., & Samuel, C. (2005). Relaxin reverses cardiac and renal fibrosis in spontaneously hypertensive rats. Hypertension, 46(2), 412 - 418. https://doi.org/10.1161/01.HYP.0000171930.00697.2f