Relaxin antagonizes hypertrophy and apoptosis in neonatal rat cardiomyocytes

Xiao-Lei Moore; Su-Ling Tan; Chen-Yi Lo; Lu Fang; Yi-Dan Su; Xiao-Ming Gao; Elizabeth A Woodcock; Roger James Summers; Geoffrey W Tregear; Ross Bathgate; Xia-Jun Du

Relaxin antagonizes hypertrophy and apoptosis in neonatal rat cardiomyocytes

Xiao-Lei Moore, Su-Ling Tan, Chen-Yi Lo, Lu Fang, Yi-Dan Su, Xiao-Ming Gao, Elizabeth A Woodcock, Roger James Summers, Geoffrey W Tregear, Ross Bathgate, Xia-Jun Du

Pharmacology

Research output: Contribution to journal › Article › Research › peer-review

72 Citations (Scopus)

Abstract

The pregnancy hormone relaxin has recently been shown to be cardio-protective. Despite its well-established anti-fibrotic actions in the heart, the effects of relaxin on cardiomyocytes (CM) remain to be determined. We investigated effects of isoform 2 of the human relaxin (H2-relaxin) on CM hypertrophy and apoptosis. In cultured neonatal rat CM, phenylephrine (50 microM) and cardiac fibroblast-conditioned medium (FCM) were used respectively to induce CM hypertrophy. The degree of hypertrophy was indicated by increased cell size, protein synthesis and gene expression of atrial natriuretic peptide (ANP). Whilst H2-relaxin (16.7 nM) alone failed to suppress hypertrophy induced by phenylephrine, it repressed the FCM-induced increase in protein synthesis by 24 (P

Original language	English
Pages (from-to)	1582 - 1589
Number of pages	8
Journal	Endocrinology
Volume	148
Issue number	4
Publication status	Published - 2007

Cite this

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title = "Relaxin antagonizes hypertrophy and apoptosis in neonatal rat cardiomyocytes",

abstract = "The pregnancy hormone relaxin has recently been shown to be cardio-protective. Despite its well-established anti-fibrotic actions in the heart, the effects of relaxin on cardiomyocytes (CM) remain to be determined. We investigated effects of isoform 2 of the human relaxin (H2-relaxin) on CM hypertrophy and apoptosis. In cultured neonatal rat CM, phenylephrine (50 microM) and cardiac fibroblast-conditioned medium (FCM) were used respectively to induce CM hypertrophy. The degree of hypertrophy was indicated by increased cell size, protein synthesis and gene expression of atrial natriuretic peptide (ANP). Whilst H2-relaxin (16.7 nM) alone failed to suppress hypertrophy induced by phenylephrine, it repressed the FCM-induced increase in protein synthesis by 24 (P",

author = "Xiao-Lei Moore and Su-Ling Tan and Chen-Yi Lo and Lu Fang and Yi-Dan Su and Xiao-Ming Gao and Woodcock, {Elizabeth A} and Summers, {Roger James} and Tregear, {Geoffrey W} and Ross Bathgate and Xia-Jun Du",

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language = "English",

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T1 - Relaxin antagonizes hypertrophy and apoptosis in neonatal rat cardiomyocytes

AU - Moore, Xiao-Lei

AU - Tan, Su-Ling

AU - Lo, Chen-Yi

AU - Fang, Lu

AU - Su, Yi-Dan

AU - Gao, Xiao-Ming

AU - Woodcock, Elizabeth A

AU - Summers, Roger James

AU - Tregear, Geoffrey W

AU - Bathgate, Ross

AU - Du, Xia-Jun

PY - 2007

Y1 - 2007

N2 - The pregnancy hormone relaxin has recently been shown to be cardio-protective. Despite its well-established anti-fibrotic actions in the heart, the effects of relaxin on cardiomyocytes (CM) remain to be determined. We investigated effects of isoform 2 of the human relaxin (H2-relaxin) on CM hypertrophy and apoptosis. In cultured neonatal rat CM, phenylephrine (50 microM) and cardiac fibroblast-conditioned medium (FCM) were used respectively to induce CM hypertrophy. The degree of hypertrophy was indicated by increased cell size, protein synthesis and gene expression of atrial natriuretic peptide (ANP). Whilst H2-relaxin (16.7 nM) alone failed to suppress hypertrophy induced by phenylephrine, it repressed the FCM-induced increase in protein synthesis by 24 (P

AB - The pregnancy hormone relaxin has recently been shown to be cardio-protective. Despite its well-established anti-fibrotic actions in the heart, the effects of relaxin on cardiomyocytes (CM) remain to be determined. We investigated effects of isoform 2 of the human relaxin (H2-relaxin) on CM hypertrophy and apoptosis. In cultured neonatal rat CM, phenylephrine (50 microM) and cardiac fibroblast-conditioned medium (FCM) were used respectively to induce CM hypertrophy. The degree of hypertrophy was indicated by increased cell size, protein synthesis and gene expression of atrial natriuretic peptide (ANP). Whilst H2-relaxin (16.7 nM) alone failed to suppress hypertrophy induced by phenylephrine, it repressed the FCM-induced increase in protein synthesis by 24 (P

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M3 - Article

SN - 0013-7227

VL - 148

SP - 1582

EP - 1589

JO - Endocrinology

JF - Endocrinology

IS - 4

ER -

Relaxin antagonizes hypertrophy and apoptosis in neonatal rat cardiomyocytes

Abstract

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