Relaxin activates multiple cAMP signaling pathway profiles in different target cells

Michelle Louise Halls, Timothy D Hewitson, Xiao-Lei Moore, Xiao-Jun Du, Ross A D Bathgate, Roger James Summers

Research output: Contribution to journalArticleResearchpeer-review

Abstract

Although RXFP1-cAMP signaling in HEK293T cell systems is now relatively well-defined, the signaling pathways activated by relaxin in its target cells and tissues are still unclear. This study aimed to examine the cAMP signaling of RXFP1 in cells that endogenously express the receptor. Seven cell types derived from various backgrounds were screened for receptor expression. Only in THP-1 cells and rat cardiac fibroblasts was there activation of the Galpha(i3)-Gbetagamma-phosphatidylinositol 3-kinase-protein kinase Czeta pathway, leading to cAMP accumulation. In all other cells there was activation of a combination of the initial pathways to affect cAMP. T-47D cells could activate only Galpha(s), whereas Colo 16 and rat renal fibroblasts from obstructed kidney could activate both Galpha(s) and Galpha(oB) pathways. Thus, the signaling pathways activated by relaxin are highly dependent upon the cell type under investigation, and this may help to explain the varied physiological responses exerted by relaxin in its different target tissues.
Original languageEnglish
Pages (from-to)108 - 111
Number of pages4
JournalAnnals of the New York Academy of Sciences
Volume1160
DOIs
Publication statusPublished - 2009

Cite this

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title = "Relaxin activates multiple cAMP signaling pathway profiles in different target cells",
abstract = "Although RXFP1-cAMP signaling in HEK293T cell systems is now relatively well-defined, the signaling pathways activated by relaxin in its target cells and tissues are still unclear. This study aimed to examine the cAMP signaling of RXFP1 in cells that endogenously express the receptor. Seven cell types derived from various backgrounds were screened for receptor expression. Only in THP-1 cells and rat cardiac fibroblasts was there activation of the Galpha(i3)-Gbetagamma-phosphatidylinositol 3-kinase-protein kinase Czeta pathway, leading to cAMP accumulation. In all other cells there was activation of a combination of the initial pathways to affect cAMP. T-47D cells could activate only Galpha(s), whereas Colo 16 and rat renal fibroblasts from obstructed kidney could activate both Galpha(s) and Galpha(oB) pathways. Thus, the signaling pathways activated by relaxin are highly dependent upon the cell type under investigation, and this may help to explain the varied physiological responses exerted by relaxin in its different target tissues.",
author = "Halls, {Michelle Louise} and Hewitson, {Timothy D} and Xiao-Lei Moore and Xiao-Jun Du and Bathgate, {Ross A D} and Summers, {Roger James}",
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language = "English",
volume = "1160",
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Relaxin activates multiple cAMP signaling pathway profiles in different target cells. / Halls, Michelle Louise; Hewitson, Timothy D; Moore, Xiao-Lei; Du, Xiao-Jun; Bathgate, Ross A D; Summers, Roger James.

In: Annals of the New York Academy of Sciences, Vol. 1160, 2009, p. 108 - 111.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Halls, Michelle Louise

AU - Hewitson, Timothy D

AU - Moore, Xiao-Lei

AU - Du, Xiao-Jun

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AU - Summers, Roger James

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AB - Although RXFP1-cAMP signaling in HEK293T cell systems is now relatively well-defined, the signaling pathways activated by relaxin in its target cells and tissues are still unclear. This study aimed to examine the cAMP signaling of RXFP1 in cells that endogenously express the receptor. Seven cell types derived from various backgrounds were screened for receptor expression. Only in THP-1 cells and rat cardiac fibroblasts was there activation of the Galpha(i3)-Gbetagamma-phosphatidylinositol 3-kinase-protein kinase Czeta pathway, leading to cAMP accumulation. In all other cells there was activation of a combination of the initial pathways to affect cAMP. T-47D cells could activate only Galpha(s), whereas Colo 16 and rat renal fibroblasts from obstructed kidney could activate both Galpha(s) and Galpha(oB) pathways. Thus, the signaling pathways activated by relaxin are highly dependent upon the cell type under investigation, and this may help to explain the varied physiological responses exerted by relaxin in its different target tissues.

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