Regulator of G-protein signalling 2 mRNA is differentially expressed in mammary epithelial subpopulations and over-expressed in the majority of breast cancers.

Matthew J Smalley, Marjan Iravani, Maria Leao, Anita Grogoriadis, Howard Kendrick, Tim Dexter, Kerry Fenwick, Joseph L Regan, Kara Louise Britt, Sarah McDonald, Christopher J Lord, Alan MacKay, Alan Ashworth

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23 Citations (Scopus)

Abstract

INTRODUCTION: To understand which signalling pathways become deregulated in breast cancer, it is necessary to identify functionally significant gene expression patterns in the stem, progenitor, transit amplifying and differentiated cells of the mammary epithelium. We have previously used the markers 33A10, CD24 and Sca-1 to identify mouse mammary epithelial cell subpopulations. We now investigate the relationship between cells expressing these markers and use gene expression microarray analysis to identify genes differentially expressed in the cell populations. METHODS: Freshly isolated primary mouse mammary epithelial cells were separated on the basis of staining with the 33A10 antibody and an alpha-Sca-1 antibody. The populations identified were profiled using gene expression microarray analysis. Gene expression patterns were confirmed on normal mouse and human mammary epithelial subpopulations and were examined in a panel of breast cancer samples and cell lines. RESULTS: Analysis of the separated populations demonstrated that Sca-1- 33A10High stained cells were estrogen receptor alpha (Esr1)- luminal epithelial cells, whereas Sca-1+ 33A10Low/- stained cells were a mix of nonepithelial cells and Esr1+ epithelial cells. Analysis...
Original languageEnglish
Pages (from-to)1 - 16
Number of pages16
JournalBreast Cancer Research
Volume9
Issue number6 (R85)
DOIs
Publication statusPublished - 2007

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