Regulation of Sertoli cell activin A and inhibin B by tumour necrosis factor a and interleukin 1a: Interaction with follicle-stimulating hormone/adenosine 3',5'-cyclic phosphate signalling

Saito Kazutaka, Wendy Winnall, Julie Muir, Mark Hedger

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    Regulation of crucial events during spermatogenesis involves dynamic changes in cytokine production and interactions across the cycle of the seminiferous epithelium. Regulation of activin A and inhibin B production by the inflammatory cytokines, tumour necrosis factor alpha (TNFalpha) and interleukin 1alpha (IL1alpha), alone and in conjunction with FSH or a cAMP analogue (dibutyryl cAMP), was examined in cultures of Sertoli cells from 20-day old rats. Both TNFalpha and IL1alpha stimulated activin A secretion and expression of its subunit (beta(A)) mRNA, and suppressed inhibin B secretion and expression of its subunit (alpha and beta(B)) mRNAs. The actions of TNFalpha and IL1alpha were opposed by FSH and dibutyryl cAMP. Both cytokines inhibited FSH/dibutyryl cAMP-stimulated inhibin B secretion and mRNA expression as well as stem cell factor mRNA expression. Both cytokines also inhibited FSH-induced cAMP production, and reduced baseline FSH receptor mRNA expression. These data highlight the reciprocal relationship that exists between FSH/cAMP signalling and inflammatory cytokine signalling pathways in the control of Sertoli cell function, and production of activin A/inhibin B in particular. It is anticipated that these interactions play important roles in the fine control of events during the cycle of the seminiferous epithelium and in the inhibition of spermatogenesis during inflammation.
    Original languageEnglish
    Pages (from-to)195 - 203
    Number of pages9
    JournalMolecular and Cellular Endocrinology
    Issue number2
    Publication statusPublished - 2011

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