TY - JOUR
T1 - Regulation of calcitonin gene-related peptide release from rat trigeminal nucleus caudalis slices in vitro
AU - Jenkins, David W.
AU - Langmead, Christopher J.
AU - Parsons, Andrew A.
AU - Strijbos, Paul J.
PY - 2004/8/19
Y1 - 2004/8/19
N2 - Calcitonin gene-related peptide (CGRP) released from trigeminal primary afferents has been implicated in the pathophysiology of migraine. Here, we have used an in vitro slice preparation to investigate its release from nerve terminals in the rat trigeminal nucleus caudalis. Extracellular-calcium dependent CGRP release was stimulated by both capsaicin and neuronal depolarization with KCl. The capsaicin (1 μM)-evoked CGRP release was blocked by capsazepine and was also attenuated in the presence of the cyclooxygenase inhibitor, indomethacin, an effect that was reversed when slices were stimulated with capsaicin in the presence of the cyclooxygenase metabolite, prostaglandin E 2. Taken together, these data further highlight the importance of prostaglandins as enhancers of neuropeptide release and suggest that CGRP released from the central terminals of trigeminal neurones has the potential to be involved in the transmission of nociceptive information of relevance to migraine headache.
AB - Calcitonin gene-related peptide (CGRP) released from trigeminal primary afferents has been implicated in the pathophysiology of migraine. Here, we have used an in vitro slice preparation to investigate its release from nerve terminals in the rat trigeminal nucleus caudalis. Extracellular-calcium dependent CGRP release was stimulated by both capsaicin and neuronal depolarization with KCl. The capsaicin (1 μM)-evoked CGRP release was blocked by capsazepine and was also attenuated in the presence of the cyclooxygenase inhibitor, indomethacin, an effect that was reversed when slices were stimulated with capsaicin in the presence of the cyclooxygenase metabolite, prostaglandin E 2. Taken together, these data further highlight the importance of prostaglandins as enhancers of neuropeptide release and suggest that CGRP released from the central terminals of trigeminal neurones has the potential to be involved in the transmission of nociceptive information of relevance to migraine headache.
KW - Calcitonin gene-related peptide
KW - Migraine
KW - Prostaglandin E
KW - Superfusion
KW - Trigeminal nucleus caudalis
UR - http://www.scopus.com/inward/record.url?scp=3543146127&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2004.05.067
DO - 10.1016/j.neulet.2004.05.067
M3 - Article
C2 - 15288426
AN - SCOPUS:3543146127
SN - 0304-3940
VL - 366
SP - 241
EP - 244
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 3
ER -