Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity

Edwin D. Hawkins, Jane Oliaro, Axel Kallies, Gabrielle T Belz, Andrew Filby, Thea Hogan, Nicole M Haynes, Kelly M Ramsbottom, Vanessa Van Ham, Tanja Kinwell, Benedict Seddon, Derek Davies, David Tarlinton, Andrew M Lew, Patrick O Humbert, Sarah M. Russell

Research output: Contribution to journalArticleResearchpeer-review

50 Citations (Scopus)

Abstract

The production of protective antibody requires effective signalling of naive B cells following encounter with antigen, and the divergence of responding B lymphocytes into distinct lineages. Polarity proteins have recently been proposed as important mediators of both the initial B cell response, and potentially of asymmetric cell division. Here we show that, although polarity proteins of the Scribble complex, Scribble, Dlg1 and Lgl1, are expressed and polarized during early B cell activation, their deficiency has no effect on the in vivo outcome of immunization or challenge with influenza infection. Furthermore, we find a striking correlation in the differentiation outcome of daughters of single founder B cells in vitro. Taken together, our results indicate that B cell differentiation does not require polarity proteins of the Scribble complex, and the findings do not support a role for asymmetric cell division in B cell activation and differentiation.

Original languageEnglish
Article number1801
JournalNature Communications
Volume4
DOIs
Publication statusPublished - 2013
Externally publishedYes

Cite this

Hawkins, E. D., Oliaro, J., Kallies, A., Belz, G. T., Filby, A., Hogan, T., Haynes, N. M., Ramsbottom, K. M., Van Ham, V., Kinwell, T., Seddon, B., Davies, D., Tarlinton, D., Lew, A. M., Humbert, P. O., & Russell, S. M. (2013). Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity. Nature Communications, 4, [1801]. https://doi.org/10.1038/ncomms2796