Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity

Edwin D. Hawkins, Jane Oliaro, Axel Kallies, Gabrielle T Belz, Andrew Filby, Thea Hogan, Nicole M Haynes, Kelly M Ramsbottom, Vanessa Van Ham, Tanja Kinwell, Benedict Seddon, Derek Davies, David Tarlinton, Andrew M Lew, Patrick O Humbert, Sarah M. Russell

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The production of protective antibody requires effective signalling of naive B cells following encounter with antigen, and the divergence of responding B lymphocytes into distinct lineages. Polarity proteins have recently been proposed as important mediators of both the initial B cell response, and potentially of asymmetric cell division. Here we show that, although polarity proteins of the Scribble complex, Scribble, Dlg1 and Lgl1, are expressed and polarized during early B cell activation, their deficiency has no effect on the in vivo outcome of immunization or challenge with influenza infection. Furthermore, we find a striking correlation in the differentiation outcome of daughters of single founder B cells in vitro. Taken together, our results indicate that B cell differentiation does not require polarity proteins of the Scribble complex, and the findings do not support a role for asymmetric cell division in B cell activation and differentiation.

Original languageEnglish
Article number1801
JournalNature Communications
Publication statusPublished - 2013
Externally publishedYes

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