Regeneration of hemopoietic precursor cells in spleen organ cultures from irradiated mice: Influence of genotype of cells injected and of the spleen microenvironment

H. Von Melchner, G. J. Lieschke

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Abstract

The regeneration of hemopoietic precursor cells (colony-forming cells, CFC) was monitored in spleen organ cultures from lethally irradiated mice injected with 107 normal syngeneic or allogeneic bone marrow cells. The important role of the microenvironment in supporting hemopoiesis was confirmed by the failure of mutant S1/S1(d) spleens to support CFC regeneration in organ cultures. However, the extent and quality of the CFC regeneration was clearly dependent on the genetic properties of the injected cells. Evidence for this was obtained from the regeneration patterns of various CFC types in organ cultured spleens derived from different mouse donor-recipient strain combinations (CBA/CBA, CBA/C57BL, CBA/BALB/c, C57BL/C57BL, C57BL/CBA, C57BL/BALB/c) that maintained the differences in the bone marrow frequency of various CFC types characteristic of the donor strain.

Original languageEnglish
Pages (from-to)906-912
Number of pages7
JournalBlood
Volume57
Issue number5
Publication statusPublished - 1981
Externally publishedYes

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