Refolding Your Protein with a Little Help from REFOLD

Jennifer Phan, Nasrin Yamout, Jason Schmidberger, Stephen Bottomley, Ashley Buckle

Research output: Chapter in Book/Report/Conference proceedingChapter (Book)Other

7 Citations (Scopus)


The expression and harvesting of proteins from insoluble inclusion bodies by solubilization and refolding is a technique commonly used in the production of recombinant proteins. Despite the importance of refolding, publications in the literature are essentially ad hoc reports consisting of a dazzling array of experimental protocols and a diverse collection of buffer cocktails. For the protein scientists, using this information to refold their protein of interest presents enormous challenges. Here, we describe some of the practical considerations in refolding and present several standard protocols. Further, we describe how refolding procedures can be designed and modified using the information in the REFOLD database (, a freely available, open repository for protocols describing the refolding and purification of recombinant proteins.
Original languageEnglish
Title of host publicationProtein Folding, Misfolding, and Disease
EditorsA F Hill, K J Barnham, S P Bottomley, R Cappai
Place of PublicationUK
Pages45 - 57
Number of pages13
ISBN (Print)9781603272216
Publication statusPublished - 2011

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