Reduced developmental competence of immature, in-vitro matured and postovulatory aged mouse oocytes following IVF and ICSI

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    Abstract

    ABSTRACT: BACKGROUND: The present study highlights basic physiological differences associated with oocyte maturation and ageing. The study explores the fertilizing capacity and resistance to injury of mouse oocytes at different stages of maturation and ageing following IVF and ICSI. Also, the study examines the developmental competence of embryos obtained from these oocytes. The outcome of the study supports views that the mouse can be a model for human IVF suggesting that utilizing in-vitro matured and failed fertilized oocytes to produce embryos mainly when limited number of oocytes is retrieved in a specific cycle, should be carefully considered. METHODS: Hybrid strain mouse oocytes were inseminated by in-vitro insemination (IVF) or intracytoplasmic sperm injection (ICSI). Oocytes groups that were used were germinal vesicle (GV) in-vitro matured metaphase II (IVM-MII), freshly ovulated MII (OV-MII), 13hrs in-vitro aged MII (13hrs-MII) and 24 hrs in-vitro aged MII (24hrs-MII). Fertilization and embryo development to the blastocyst stage were monitored up to 5 days in culture for IVF and ICSI zygotes. Sperm head decondensation and pronuclear formation were examined up to 9hrs in oocytes following ICSI. Apoptotic events in blocked embryos were examined using the TUNNEL assay. Differences between females for the number and quality of GV and OV-MII oocytes were examined by ANOVA analyses. Differences in survival after ICSI, fertilization by IVF and ICSI and embryo development were analysed by Chi-square test with Yates correction. RESULTS: No differences in number and quality of oocytes were identified between females. The findings suggest that inability of GV oocytes to participate in fertilization and embryo development initiates primarily from their inability to support initial post fertilization...
    Original languageEnglish
    Pages (from-to)1 - 58
    Number of pages58
    JournalReproductive Biology and Endocrinology
    Volume6
    Issue number1
    Publication statusPublished - 2008

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