TY - JOUR
T1 - Red blood cell age determines the impact of storage and leukocyte burden on cell adhesion molecules, glycophorin A and the release of annexin V
AU - Sparrow, Rosemary L.
AU - Healey, Geraldine
AU - Patton, Katherine A.
AU - Veale, Margaret F.
N1 - Funding Information:
This work is supported by grants from the National Australia Bank—Australian Red Cross Blood Service Research Fund and the Australasian Society of Blood Transfusion Research Fund. We thank Dr. Angela Anniss for assistance and Associate Professor Neil Boyce for helpful discussion and review of the manuscript.
Copyright:
Copyright 2008 Elsevier B.V., All rights reserved.
PY - 2006/2
Y1 - 2006/2
N2 - The influence of the age of the red blood cell (RBC) within its 120-day lifecycle at the time of blood donation on the RBC storage lesion is not well understood. Expression of cell adhesion molecules (CAMs) (CD44, CD47, CD58 and CD147), glycophorin A (GPA) and phosphatidylserine (PS) on young and old RBCs density separated prior to storage of the RBC concentrate was determined by flow cytometry. Older RBCs showed significantly reduced expression of GPA throughout storage and CD44 and CD147 from Day 28 onwards compared to young RBCs. Storage in the presence of leukocytes caused a significant decline in the expression of CD44, CD58, CD147 and GPA, whereas RBCs that were pre-storage leukocyte depleted maintained a relatively consistent level of expression throughout storage. PS was not detected at the external RBC membrane of young or old RBCs during storage. Increased levels of annexin V were detected in the supernatant of RBCs stored in the presence of leukocytes, with significantly greater supernatant levels found for old RBCs compared to young RBCs. These findings provide new insight into the RBC storage lesion and indicate that RBC age at the time of donation impacts upon the quality of stored RBC concentrates.
AB - The influence of the age of the red blood cell (RBC) within its 120-day lifecycle at the time of blood donation on the RBC storage lesion is not well understood. Expression of cell adhesion molecules (CAMs) (CD44, CD47, CD58 and CD147), glycophorin A (GPA) and phosphatidylserine (PS) on young and old RBCs density separated prior to storage of the RBC concentrate was determined by flow cytometry. Older RBCs showed significantly reduced expression of GPA throughout storage and CD44 and CD147 from Day 28 onwards compared to young RBCs. Storage in the presence of leukocytes caused a significant decline in the expression of CD44, CD58, CD147 and GPA, whereas RBCs that were pre-storage leukocyte depleted maintained a relatively consistent level of expression throughout storage. PS was not detected at the external RBC membrane of young or old RBCs during storage. Increased levels of annexin V were detected in the supernatant of RBCs stored in the presence of leukocytes, with significantly greater supernatant levels found for old RBCs compared to young RBCs. These findings provide new insight into the RBC storage lesion and indicate that RBC age at the time of donation impacts upon the quality of stored RBC concentrates.
KW - Age
KW - Annexin V
KW - Cell adhesion molecules
KW - Glycophorin A
KW - Phosphatidylserine
KW - RBC storage lesion
UR - http://www.scopus.com/inward/record.url?scp=32344450806&partnerID=8YFLogxK
U2 - 10.1016/j.transci.2005.09.006
DO - 10.1016/j.transci.2005.09.006
M3 - Article
C2 - 16377250
AN - SCOPUS:32344450806
SN - 1473-0502
VL - 34
SP - 15
EP - 23
JO - Transfusion and Apheresis Science
JF - Transfusion and Apheresis Science
IS - 1
ER -