Reconstitution of the initial steps of mitochondrial protein import

Naomi Hachiya, Katsuyoshi Mihara, Kitaru Suda, Martin Horst, Gottfried Schatz, Trevor Lithgow

Research output: Contribution to journalLetterResearchpeer-review

147 Citations (Scopus)

Abstract

We have reconstituted the initial steps of mitochondrial protein import with a purified precursor protein, a purified, ATP-dependent, cytosolic chaperone selective for mitochondrial precursors (mitochondrial import stimulating factor; MSF), and either intact mitochondria or intact or solubilized mitochondrial outer membranes. We show that the precursor-MSF complex first binds to the Mas37p/Mas70p subunits of the mitochondrial import receptor. After ATP-dependent release of MSF, the precursor is transferred from Mas37p/Mas70p to the Mas20p/Mas22p sub-units of the receptor, and finally delivered to the import channel in the outer membrane. Import in the absence of the MSF bypasses Mas37p/Mas70p. The ATP-mediated transfer of a precursor from MSF to specific subunits of the import receptor is similar to the GTP-mediated transfer of precursors from the signal recognition particle to its receptor on the endoplasmic reticulum.

Original languageEnglish
Pages (from-to)705-709
Number of pages5
JournalNature
Volume376
Issue number6542
DOIs
Publication statusPublished - 24 Aug 1995
Externally publishedYes

Cite this

Hachiya, N., Mihara, K., Suda, K., Horst, M., Schatz, G., & Lithgow, T. (1995). Reconstitution of the initial steps of mitochondrial protein import. Nature, 376(6542), 705-709. https://doi.org/10.1038/376705a0
Hachiya, Naomi ; Mihara, Katsuyoshi ; Suda, Kitaru ; Horst, Martin ; Schatz, Gottfried ; Lithgow, Trevor. / Reconstitution of the initial steps of mitochondrial protein import. In: Nature. 1995 ; Vol. 376, No. 6542. pp. 705-709.
@article{30069a2fb3db4ce6865af846b9f6c757,
title = "Reconstitution of the initial steps of mitochondrial protein import",
abstract = "We have reconstituted the initial steps of mitochondrial protein import with a purified precursor protein, a purified, ATP-dependent, cytosolic chaperone selective for mitochondrial precursors (mitochondrial import stimulating factor; MSF), and either intact mitochondria or intact or solubilized mitochondrial outer membranes. We show that the precursor-MSF complex first binds to the Mas37p/Mas70p subunits of the mitochondrial import receptor. After ATP-dependent release of MSF, the precursor is transferred from Mas37p/Mas70p to the Mas20p/Mas22p sub-units of the receptor, and finally delivered to the import channel in the outer membrane. Import in the absence of the MSF bypasses Mas37p/Mas70p. The ATP-mediated transfer of a precursor from MSF to specific subunits of the import receptor is similar to the GTP-mediated transfer of precursors from the signal recognition particle to its receptor on the endoplasmic reticulum.",
author = "Naomi Hachiya and Katsuyoshi Mihara and Kitaru Suda and Martin Horst and Gottfried Schatz and Trevor Lithgow",
year = "1995",
month = "8",
day = "24",
doi = "10.1038/376705a0",
language = "English",
volume = "376",
pages = "705--709",
journal = "Nature",
issn = "0028-0836",
publisher = "Nature Publishing Group",
number = "6542",

}

Hachiya, N, Mihara, K, Suda, K, Horst, M, Schatz, G & Lithgow, T 1995, 'Reconstitution of the initial steps of mitochondrial protein import', Nature, vol. 376, no. 6542, pp. 705-709. https://doi.org/10.1038/376705a0

Reconstitution of the initial steps of mitochondrial protein import. / Hachiya, Naomi; Mihara, Katsuyoshi; Suda, Kitaru; Horst, Martin; Schatz, Gottfried; Lithgow, Trevor.

In: Nature, Vol. 376, No. 6542, 24.08.1995, p. 705-709.

Research output: Contribution to journalLetterResearchpeer-review

TY - JOUR

T1 - Reconstitution of the initial steps of mitochondrial protein import

AU - Hachiya, Naomi

AU - Mihara, Katsuyoshi

AU - Suda, Kitaru

AU - Horst, Martin

AU - Schatz, Gottfried

AU - Lithgow, Trevor

PY - 1995/8/24

Y1 - 1995/8/24

N2 - We have reconstituted the initial steps of mitochondrial protein import with a purified precursor protein, a purified, ATP-dependent, cytosolic chaperone selective for mitochondrial precursors (mitochondrial import stimulating factor; MSF), and either intact mitochondria or intact or solubilized mitochondrial outer membranes. We show that the precursor-MSF complex first binds to the Mas37p/Mas70p subunits of the mitochondrial import receptor. After ATP-dependent release of MSF, the precursor is transferred from Mas37p/Mas70p to the Mas20p/Mas22p sub-units of the receptor, and finally delivered to the import channel in the outer membrane. Import in the absence of the MSF bypasses Mas37p/Mas70p. The ATP-mediated transfer of a precursor from MSF to specific subunits of the import receptor is similar to the GTP-mediated transfer of precursors from the signal recognition particle to its receptor on the endoplasmic reticulum.

AB - We have reconstituted the initial steps of mitochondrial protein import with a purified precursor protein, a purified, ATP-dependent, cytosolic chaperone selective for mitochondrial precursors (mitochondrial import stimulating factor; MSF), and either intact mitochondria or intact or solubilized mitochondrial outer membranes. We show that the precursor-MSF complex first binds to the Mas37p/Mas70p subunits of the mitochondrial import receptor. After ATP-dependent release of MSF, the precursor is transferred from Mas37p/Mas70p to the Mas20p/Mas22p sub-units of the receptor, and finally delivered to the import channel in the outer membrane. Import in the absence of the MSF bypasses Mas37p/Mas70p. The ATP-mediated transfer of a precursor from MSF to specific subunits of the import receptor is similar to the GTP-mediated transfer of precursors from the signal recognition particle to its receptor on the endoplasmic reticulum.

UR - http://www.scopus.com/inward/record.url?scp=0029096887&partnerID=8YFLogxK

U2 - 10.1038/376705a0

DO - 10.1038/376705a0

M3 - Letter

VL - 376

SP - 705

EP - 709

JO - Nature

JF - Nature

SN - 0028-0836

IS - 6542

ER -

Hachiya N, Mihara K, Suda K, Horst M, Schatz G, Lithgow T. Reconstitution of the initial steps of mitochondrial protein import. Nature. 1995 Aug 24;376(6542):705-709. https://doi.org/10.1038/376705a0