Recombination between linear double-stranded DNA substrates in vivo

Kumaran Narayanan; Edmund Ui Hang Sim; Nikolai V. Ravin; Choon Weng Lee

doi:10.1016/j.ab.2009.01.015

Recombination between linear double-stranded DNA substrates in vivo

Kumaran Narayanan, Edmund Ui Hang Sim, Nikolai V. Ravin, Choon Weng Lee

Research output: Contribution to journal › Article › Research › peer-review

12 Citations (Scopus)

Abstract

Recombineering technology in Escherichia coli enables targeting of linear donor DNA to circular recipient DNA using short shared homology sequences. In this work, we demonstrate that recombineering is also able to support recombination between a pair of linear DNA substrates (linear/linear recombineering) in vivo in E. coli. Linear DNA up to 100 kb is accurately modified and remains intact without undergoing rearrangements after recombination. This system will be valuable for direct in vivo manipulation of large linear DNA including the N15 and PY54 prophages and linear animal viruses, and for assembly of linear constructs as artificial chromosome vectors.

Original language	English
Pages (from-to)	139-141
Number of pages	3
Journal	Analytical Biochemistry
Volume	387
Issue number	1
DOIs	https://doi.org/10.1016/j.ab.2009.01.015
Publication status	Published - 1 Apr 2009
Externally published	Yes

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10.1016/j.ab.2009.01.015

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AU - Sim, Edmund Ui Hang

AU - Ravin, Nikolai V.

AU - Lee, Choon Weng

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Recombination between linear double-stranded DNA substrates in vivo

Abstract

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