Recombinant type I regulatory subunit of the cAMP-dependent protein kinase is biologically active

Stephan Oehen, Jo Anne Parsons, David A. Jans, Brian A. Hemmings

Research output: Contribution to journalArticleResearchpeer-review

Abstract

The cDNA for the porcine type I regulatory subunit (RI) of the cAMP-dependent protein kinase (cAMP-PK) was cloned into two different bacterial expression vectors: pKK223 and pUC18. Recombinant RI was produced by bacteria transformed with either construct, and purified by affinity chromatography. Both the native RI from the pKK223 construct and the RI with an amino terminal extension of eight amino acids from the pUC18 construct were found to be completely native with regard to inhibition of the catalytic subunit activity and cAMP binding.

Original languageEnglish
Pages (from-to)47-51
Number of pages5
JournalFEBS Letters
Volume220
Issue number1
DOIs
Publication statusPublished - 10 Aug 1987
Externally publishedYes

Keywords

  • Bacterial expression vector
  • lac promoter
  • Plasmid pKK223
  • Plasmid pUC18
  • Protein kinase

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