TY - JOUR
T1 - Recombinant soluble human CD69 dimer produced in Escherichia coli
T2 - Reevaluation of saccharide binding
AU - Childs, Robert A.
AU - Galustian, Christine
AU - Lawson, Alexander M.
AU - Dougan, Gordon
AU - Benwell, Karen
AU - Frankel, Gad
AU - Feizi, Ten
PY - 1999/12/9
Y1 - 1999/12/9
N2 - We reevaluate here an earlier report of monosaccharide binding by the C-type lectin-like, leukocyte surface protein CD69 in the form of a recombinant soluble dimer, and we examine polysaccharide binding by the protein. We have expressed in Escherichia coli a new construct of the extracellular part (Q65-K199) of human CD69. We describe the folding in vitro to produce, in good yield, the protein in a soluble, disulphide-linked, dimeric form, and the results of binding experiments with monosaccharides: glucose, galactose, mannose, fucose, N-acetylglucosamine, and N-acetylgalactosamine, linked to bovine serum albumin. Monosaccharide-binding signals are not detectable. Among the polysaccharides, heparin, chondroitin sulphates A, B, and C, fucoidan, and dextran sulphate, CD69 dimer gives a weak binding signal with fucoidan.
AB - We reevaluate here an earlier report of monosaccharide binding by the C-type lectin-like, leukocyte surface protein CD69 in the form of a recombinant soluble dimer, and we examine polysaccharide binding by the protein. We have expressed in Escherichia coli a new construct of the extracellular part (Q65-K199) of human CD69. We describe the folding in vitro to produce, in good yield, the protein in a soluble, disulphide-linked, dimeric form, and the results of binding experiments with monosaccharides: glucose, galactose, mannose, fucose, N-acetylglucosamine, and N-acetylgalactosamine, linked to bovine serum albumin. Monosaccharide-binding signals are not detectable. Among the polysaccharides, heparin, chondroitin sulphates A, B, and C, fucoidan, and dextran sulphate, CD69 dimer gives a weak binding signal with fucoidan.
UR - http://www.scopus.com/inward/record.url?scp=0033539913&partnerID=8YFLogxK
U2 - 10.1006/bbrc.1999.1762
DO - 10.1006/bbrc.1999.1762
M3 - Article
C2 - 10581158
AN - SCOPUS:0033539913
SN - 0006-291X
VL - 266
SP - 19
EP - 23
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 1
ER -