Recombinant NY-ESO-1 cancer antigen: Production and purification under cGMP conditions

R. Murphy; S. Green; G. Ritter; L. Cohen; D. Ryan; W. Woods; M. Rubira; J. Cebon; I. D. Davis; A. Sjolander; A. Kypridis; H. Kalnins; M. McNamara; M. B. Moloney; J. Ackland; G. Cartwright; J. Rood; G. Dumsday; K. Healey; D. Maher; E. Maraskovsky; Y. T. Chen; E. W. Hoffman; L. J. Old; A. M. Scott

doi:10.1081/PB-200054732

Recombinant NY-ESO-1 cancer antigen: Production and purification under cGMP conditions

R. Murphy, S. Green, G. Ritter, L. Cohen, D. Ryan, W. Woods, M. Rubira, J. Cebon, I. D. Davis, A. Sjolander, A. Kypridis, H. Kalnins, M. McNamara, M. B. Moloney, J. Ackland, G. Cartwright, J. Rood, G. Dumsday, K. Healey, D. MaherE. Maraskovsky, Y. T. Chen, E. W. Hoffman, L. J. Old, A. M. Scott

Research output: Contribution to journal › Article › Research › peer-review

32 Citations (Scopus)

Abstract

The cancer-testis antigen, NY-ESO-1, has been engineered into a bacterial expression plasmid which incorporates a His₆-tag. The plasmid was transfected into E. coli strain BL21 and Master and Working cell banks generated from this expression system. Three 15-litre fermentations were performed under cGMP (code of Good Manufacturing Practice) conditions and the crude NY-ESO-1 tagged protein isolated as solubilised inclusion bodies. A three-step cGMP chromatography process (immobilised metal affinity, anion exchange, and hydrophobic interaction) was utilised to purify the protein. The purified NY-ESO-1 is being used in early stage human cancer vaccine trials in Australia and the U.S.A.

Original language	English
Pages (from-to)	119-134
Number of pages	16
Journal	Preparative Biochemistry & Biotechnology
Volume	35
Issue number	2
DOIs	https://doi.org/10.1081/PB-200054732
Publication status	Published - 10 May 2005

Keywords

Cancer-testis antigen
cGMP
NY-ESO-1
Recombinant protein

Access to Document

10.1081/PB-200054732

Link to publication in Scopus

Cite this

Murphy, R., Green, S., Ritter, G., Cohen, L., Ryan, D., Woods, W., Rubira, M., Cebon, J., Davis, I. D., Sjolander, A., Kypridis, A., Kalnins, H., McNamara, M., Moloney, M. B., Ackland, J., Cartwright, G., Rood, J., Dumsday, G., Healey, K., ... Scott, A. M. (2005). Recombinant NY-ESO-1 cancer antigen: Production and purification under cGMP conditions. Preparative Biochemistry & Biotechnology, 35(2), 119-134. https://doi.org/10.1081/PB-200054732

Murphy, R. ; Green, S. ; Ritter, G. ; Cohen, L. ; Ryan, D. ; Woods, W. ; Rubira, M. ; Cebon, J. ; Davis, I. D. ; Sjolander, A. ; Kypridis, A. ; Kalnins, H. ; McNamara, M. ; Moloney, M. B. ; Ackland, J. ; Cartwright, G. ; Rood, J. ; Dumsday, G. ; Healey, K. ; Maher, D. ; Maraskovsky, E. ; Chen, Y. T. ; Hoffman, E. W. ; Old, L. J. ; Scott, A. M. / Recombinant NY-ESO-1 cancer antigen : Production and purification under cGMP conditions. In: Preparative Biochemistry & Biotechnology. 2005 ; Vol. 35, No. 2. pp. 119-134.

@article{e0176549838b499f893cc23ddc5f097e,

title = "Recombinant NY-ESO-1 cancer antigen: Production and purification under cGMP conditions",

abstract = "The cancer-testis antigen, NY-ESO-1, has been engineered into a bacterial expression plasmid which incorporates a His6-tag. The plasmid was transfected into E. coli strain BL21 and Master and Working cell banks generated from this expression system. Three 15-litre fermentations were performed under cGMP (code of Good Manufacturing Practice) conditions and the crude NY-ESO-1 tagged protein isolated as solubilised inclusion bodies. A three-step cGMP chromatography process (immobilised metal affinity, anion exchange, and hydrophobic interaction) was utilised to purify the protein. The purified NY-ESO-1 is being used in early stage human cancer vaccine trials in Australia and the U.S.A.",

keywords = "Cancer-testis antigen, cGMP, NY-ESO-1, Recombinant protein",

author = "R. Murphy and S. Green and G. Ritter and L. Cohen and D. Ryan and W. Woods and M. Rubira and J. Cebon and Davis, {I. D.} and A. Sjolander and A. Kypridis and H. Kalnins and M. McNamara and Moloney, {M. B.} and J. Ackland and G. Cartwright and J. Rood and G. Dumsday and K. Healey and D. Maher and E. Maraskovsky and Chen, {Y. T.} and Hoffman, {E. W.} and Old, {L. J.} and Scott, {A. M.}",

year = "2005",

month = may,

day = "10",

doi = "10.1081/PB-200054732",

language = "English",

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Murphy, R, Green, S, Ritter, G, Cohen, L, Ryan, D, Woods, W, Rubira, M, Cebon, J, Davis, ID, Sjolander, A, Kypridis, A, Kalnins, H, McNamara, M, Moloney, MB, Ackland, J, Cartwright, G, Rood, J, Dumsday, G, Healey, K, Maher, D, Maraskovsky, E, Chen, YT, Hoffman, EW, Old, LJ & Scott, AM 2005, 'Recombinant NY-ESO-1 cancer antigen: Production and purification under cGMP conditions', Preparative Biochemistry & Biotechnology, vol. 35, no. 2, pp. 119-134. https://doi.org/10.1081/PB-200054732

Recombinant NY-ESO-1 cancer antigen : Production and purification under cGMP conditions. / Murphy, R.; Green, S.; Ritter, G.; Cohen, L.; Ryan, D.; Woods, W.; Rubira, M.; Cebon, J.; Davis, I. D.; Sjolander, A.; Kypridis, A.; Kalnins, H.; McNamara, M.; Moloney, M. B.; Ackland, J.; Cartwright, G.; Rood, J.; Dumsday, G.; Healey, K.; Maher, D.; Maraskovsky, E.; Chen, Y. T.; Hoffman, E. W.; Old, L. J.; Scott, A. M.

In: Preparative Biochemistry & Biotechnology, Vol. 35, No. 2, 10.05.2005, p. 119-134.

Research output: Contribution to journal › Article › Research › peer-review

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T1 - Recombinant NY-ESO-1 cancer antigen

T2 - Production and purification under cGMP conditions

AU - Murphy, R.

AU - Green, S.

AU - Ritter, G.

AU - Cohen, L.

AU - Ryan, D.

AU - Woods, W.

AU - Rubira, M.

AU - Cebon, J.

AU - Davis, I. D.

AU - Sjolander, A.

AU - Kypridis, A.

AU - Kalnins, H.

AU - McNamara, M.

AU - Moloney, M. B.

AU - Ackland, J.

AU - Cartwright, G.

AU - Rood, J.

AU - Dumsday, G.

AU - Healey, K.

AU - Maher, D.

AU - Maraskovsky, E.

AU - Chen, Y. T.

AU - Hoffman, E. W.

AU - Old, L. J.

AU - Scott, A. M.

PY - 2005/5/10

Y1 - 2005/5/10

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KW - Cancer-testis antigen

KW - cGMP

KW - NY-ESO-1

KW - Recombinant protein

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JF - Preparative Biochemistry & Biotechnology

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