Recognition of CD1d-sulfatide mediated by a type II natural killer T cell antigen receptor

Onisha G Patel, Daniel G Pellicci, Stephanie Gras, Maria L Sandoval Romero, Adam P Uldrich, Thierry Mallevaey, Andrew J Clarke, Jerome Le Nours, Alex Theodossis, Susanna L Cardell, Laurent Gapin, Dale I Godfrey, Jamie Rossjohn

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105 Citations (Scopus)


Natural killer T cells (NKT cells) are divided into type I and type II subsets on the basis of differences in their T cell antigen receptor (TCR) repertoire and CD1d-antigen specificity. Although the mode by which type I NKT cell TCRs recognize CD1d-antigen has been established, how type II NKT cell TCRs engage CD1d-antigen is unknown. Here we provide a basis for how a type II NKT cell TCR, XV19, recognized CD1d-sulfatide. The XV19 TCR bound orthogonally above the A pocket of CD1d, in contrast to the parallel docking of type I NKT cell TCRs over the F pocket of CD1d. At the XV19 TCR-CD1d-sulfatide interface, the TCRalpha and TCRbeta chains sat centrally on CD1d, where the malleable CDR3 loops dominated interactions with CD1d-sulfatide. Accordingly, we highlight the diverse mechanisms by which NKT cell TCRs can bind CD1d and account for the distinct antigen specificity of type II NKT cells.
Original languageEnglish
Pages (from-to)857 - 863
Number of pages7
JournalNature Immunology
Issue number9
Publication statusPublished - 2012

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