TY - JOUR
T1 - Recoding of the stop codon UGA to glycine by a BD1-5/SN-2 bacterium and niche partitioning between Alpha- and Gammaproteobacteria in a tidal sediment microbial community naturally selected in a laboratory chemostat
AU - Hanke, Anna
AU - Hamann, Emmo
AU - Sharma, Ritin
AU - Geelhoed, Jeanine S.
AU - Hargesheimer, Theresa
AU - Kraft, Beate
AU - Meyer, Volker
AU - Lenk, Sabine
AU - Osmers, Harald
AU - Wu, Rong
AU - Makinwa, Kofi
AU - Hettich, Robert L.
AU - Banfield, Jillian F.
AU - Tegetmeyer, Halina E.
AU - Strous, Marc
PY - 2014/5/16
Y1 - 2014/5/16
N2 - Sandy coastal sediments are global hotspots for microbial mineralization of organic matter and denitrification. These sediments are characterized by advective porewater flow, tidal cycling and an active and complex microbial community. Metagenomic sequencing of microbial communities sampled from such sediments showed that potential sulfur oxidizing Gammaproteobacteria and members of the enigmatic BD1-5/SN-2 candidate phylum were abundant in situ (>10% and ̃2% respectively). By mimicking the dynamic oxic/anoxic environmental conditions of the sediment in a laboratory chemostat, a simplified microbial community was selected from the more complex inoculum. Metagenomics, proteomics and fluorescence in situ hybridization showed that this simplified community contained both a potential sulfur oxidizing Gammaproteobacteria (at 24 ± 2% abundance) and a member of the BD1-5/SN-2 candidate phylum (at 7 ± 6% abundance). Despite the abundant supply of organic substrates to the chemostat, proteomic analysis suggested that the selected gammaproteobacterium grew partially autotrophically and performed hydrogen/formate oxidation. The enrichment of a member of the BD1-5/SN-2 candidate phylum enabled, for the first time, direct microscopic observation by fluorescent in situ hybridization and the experimental validation of the previously predicted translation of the stop codon UGA into glycine.
AB - Sandy coastal sediments are global hotspots for microbial mineralization of organic matter and denitrification. These sediments are characterized by advective porewater flow, tidal cycling and an active and complex microbial community. Metagenomic sequencing of microbial communities sampled from such sediments showed that potential sulfur oxidizing Gammaproteobacteria and members of the enigmatic BD1-5/SN-2 candidate phylum were abundant in situ (>10% and ̃2% respectively). By mimicking the dynamic oxic/anoxic environmental conditions of the sediment in a laboratory chemostat, a simplified microbial community was selected from the more complex inoculum. Metagenomics, proteomics and fluorescence in situ hybridization showed that this simplified community contained both a potential sulfur oxidizing Gammaproteobacteria (at 24 ± 2% abundance) and a member of the BD1-5/SN-2 candidate phylum (at 7 ± 6% abundance). Despite the abundant supply of organic substrates to the chemostat, proteomic analysis suggested that the selected gammaproteobacterium grew partially autotrophically and performed hydrogen/formate oxidation. The enrichment of a member of the BD1-5/SN-2 candidate phylum enabled, for the first time, direct microscopic observation by fluorescent in situ hybridization and the experimental validation of the previously predicted translation of the stop codon UGA into glycine.
KW - Chemostat
KW - Continuous culture
KW - Enrichment
KW - Maritimibacter
KW - Roseobacter
KW - Stop codon
UR - http://www.scopus.com/inward/record.url?scp=84904861504&partnerID=8YFLogxK
U2 - 10.3389/fmicb.2014.00231
DO - 10.3389/fmicb.2014.00231
M3 - Article
AN - SCOPUS:84904861504
SN - 1664-302X
VL - 5
JO - Frontiers in Microbiology
JF - Frontiers in Microbiology
IS - MAY
M1 - 231
ER -