Recent Developments in Recombinant Proteins for Diagnosis of Human Fascioliasis

Abolfazl Mirzadeh, Farid Jafarihaghighi, Elham Kazemirad, Shokouh Shahrokhi Sabzevar, Mohammad Hossein Tanipour, Mehdi Ardjmand

Research output: Contribution to journalReview ArticleResearchpeer-review

6 Citations (Scopus)

Abstract

Fascioliasis is an important neglected tropical disease that causes severe injury to the bile ducts and liver. Therefore, a rapid and accurate method for detection of Fasciola hepatica infection plays a vital role in early treatment. Currently, the diagnosis of fascioliasis is mainly conducted via serological tests using the excretory/secretory (E/S) products, which might cross-react with antigens from other helminth parasitic diseases. Hence, the development of serodiagnosis test using recombinant antigens may contribute to differentiate fascioliasis from other helminth infections. In the past 20 years, many attempts have been made to exert different F. hepatica recombinant antigens to obtain a well-established standard assay with high accuracy. In this review, we address recent studies that refer to the development of serodiagnosis tests for diagnosis of human fascioliasis based on the candidate recombinant antigens produced by different approaches. Meanwhile, in the present review, some main factors have been highlighted to improve the accuracy of diagnostic tests such as the effect of refolding methods to recover antigens’ tertiary structure as well as applying a mixture of recombinant antigens with the highest sensitivity and specificity to improve the accuracy of diagnostic tests.

Original languageEnglish
Pages (from-to)13-25
Number of pages13
JournalActa Parasitologica
Volume66
Issue number1
DOIs
Publication statusPublished - Mar 2021
Externally publishedYes

Keywords

  • Expression systems
  • Human fascioliasis
  • Purification methods
  • Recombinant antigens
  • Serodiagnosis

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