Rearrangements of large-insert T-DNAs in transgenic rice

Akiko Nakano, Go Suzuki, Maki Yamamoto, Kym Turnbull, Sadequr Rahman, Yasuhiko Mukai

Research output: Contribution to journalArticleResearchpeer-review

24 Citations (Scopus)

Abstract

Introduction of large-DNA fragments into cereals by Agrobacterium-mediated transformation is a useful technique for map-based cloning and molecular breeding. However, little is known about the organization and stability of large fragments of foreign DNA introduced into plant genomes. In this study, we produced transgenic rice plants by Agrobacterium-mediated transformation with a large-insert T-DNA containing a 92-kb region of the wheat genome. The structures of the T-DNA in four independent transgenic lines were visualized by fluorescence in situ hybridization on extended DNA fibers (fiber FISH). By using this cytogenetic technique, we showed that rearrangements of the large-insert T-DNA, involving duplication, deletion and insertion, had occurred in all four lines. Deletion of long stretches of the large-insert DNA was also observed in Agrobacterium.

Original languageEnglish
Pages (from-to)123-129
Number of pages7
JournalMolecular Genetics and Genomics
Volume273
Issue number2
DOIs
Publication statusPublished - Apr 2005
Externally publishedYes

Keywords

  • Agrobacterium-mediated transformation
  • DNA rearrangement
  • Fluorescence in situ hybridization on extended DNA fibers (fiber FISH)
  • Large T-DNA inserts
  • Oryza sativa

Cite this