TY - JOUR
T1 - Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish
AU - Palha, Nuno
AU - Guivel-Benhassine, Florence
AU - Briolat, Valerie
AU - Lutfalla, Georges
AU - Sourisseau, Marion
AU - Ellett, Felix
AU - Wang, Chieh-Huei
AU - Lieschke, Graham
AU - Herbomel, Philippe
AU - Schwartz, Olivier
AU - Levraud, Jean-Pierre
PY - 2013
Y1 - 2013
N2 - Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe a novel CHIKV infection model in zebrafish, where viral spread was live-imaged in the whole body up to cellular resolution. Infected cells emerged in various organs in one principal wave with a median appearance time of approximately 14 hours post infection. Timing of infected cell death was organ dependent, leading to a shift of CHIKV localization towards the brain. As in mammals, CHIKV infection triggered a strong type-I interferon (IFN) response, critical for survival. IFN was mainly expressed by neutrophils and hepatocytes. Cell type specific ablation experiments further demonstrated that neutrophils play a crucial, unexpected role in CHIKV containment. Altogether, our results show that the zebrafish represents a novel valuable model to dynamically visualize replication, pathogenesis and host responses to a human virus.
AB - Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe a novel CHIKV infection model in zebrafish, where viral spread was live-imaged in the whole body up to cellular resolution. Infected cells emerged in various organs in one principal wave with a median appearance time of approximately 14 hours post infection. Timing of infected cell death was organ dependent, leading to a shift of CHIKV localization towards the brain. As in mammals, CHIKV infection triggered a strong type-I interferon (IFN) response, critical for survival. IFN was mainly expressed by neutrophils and hepatocytes. Cell type specific ablation experiments further demonstrated that neutrophils play a crucial, unexpected role in CHIKV containment. Altogether, our results show that the zebrafish represents a novel valuable model to dynamically visualize replication, pathogenesis and host responses to a human virus.
UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764224/pdf/ppat.1003619.pdf
U2 - 10.1371/journal.ppat.1003619
DO - 10.1371/journal.ppat.1003619
M3 - Article
SN - 1553-7366
VL - 9
SP - 1
EP - 15
JO - PLoS Pathogens
JF - PLoS Pathogens
IS - 9 (Art. No.: e1003619)
ER -
