Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish

Nuno Palha, Florence Guivel-Benhassine, Valerie Briolat, Georges Lutfalla, Marion Sourisseau, Felix Ellett, Chieh-Huei Wang, Graham Lieschke, Philippe Herbomel, Olivier Schwartz, Jean-Pierre Levraud

Research output: Contribution to journalArticleResearchpeer-review

77 Citations (Scopus)

Abstract

Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe a novel CHIKV infection model in zebrafish, where viral spread was live-imaged in the whole body up to cellular resolution. Infected cells emerged in various organs in one principal wave with a median appearance time of approximately 14 hours post infection. Timing of infected cell death was organ dependent, leading to a shift of CHIKV localization towards the brain. As in mammals, CHIKV infection triggered a strong type-I interferon (IFN) response, critical for survival. IFN was mainly expressed by neutrophils and hepatocytes. Cell type specific ablation experiments further demonstrated that neutrophils play a crucial, unexpected role in CHIKV containment. Altogether, our results show that the zebrafish represents a novel valuable model to dynamically visualize replication, pathogenesis and host responses to a human virus.
Original languageEnglish
Pages (from-to)1 - 15
Number of pages15
JournalPLoS Pathogens
Volume9
Issue number9 (Art. No.: e1003619)
DOIs
Publication statusPublished - 2013

Cite this

Palha, N., Guivel-Benhassine, F., Briolat, V., Lutfalla, G., Sourisseau, M., Ellett, F., ... Levraud, J-P. (2013). Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish. PLoS Pathogens, 9(9 (Art. No.: e1003619)), 1 - 15. https://doi.org/10.1371/journal.ppat.1003619
Palha, Nuno ; Guivel-Benhassine, Florence ; Briolat, Valerie ; Lutfalla, Georges ; Sourisseau, Marion ; Ellett, Felix ; Wang, Chieh-Huei ; Lieschke, Graham ; Herbomel, Philippe ; Schwartz, Olivier ; Levraud, Jean-Pierre. / Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish. In: PLoS Pathogens. 2013 ; Vol. 9, No. 9 (Art. No.: e1003619). pp. 1 - 15.
@article{63c6878c9f72468dabc2de1c287ce725,
title = "Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish",
abstract = "Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe a novel CHIKV infection model in zebrafish, where viral spread was live-imaged in the whole body up to cellular resolution. Infected cells emerged in various organs in one principal wave with a median appearance time of approximately 14 hours post infection. Timing of infected cell death was organ dependent, leading to a shift of CHIKV localization towards the brain. As in mammals, CHIKV infection triggered a strong type-I interferon (IFN) response, critical for survival. IFN was mainly expressed by neutrophils and hepatocytes. Cell type specific ablation experiments further demonstrated that neutrophils play a crucial, unexpected role in CHIKV containment. Altogether, our results show that the zebrafish represents a novel valuable model to dynamically visualize replication, pathogenesis and host responses to a human virus.",
author = "Nuno Palha and Florence Guivel-Benhassine and Valerie Briolat and Georges Lutfalla and Marion Sourisseau and Felix Ellett and Chieh-Huei Wang and Graham Lieschke and Philippe Herbomel and Olivier Schwartz and Jean-Pierre Levraud",
year = "2013",
doi = "10.1371/journal.ppat.1003619",
language = "English",
volume = "9",
pages = "1 -- 15",
journal = "PLoS Pathogens",
issn = "1553-7366",
publisher = "Public Library of Science",
number = "9 (Art. No.: e1003619)",

}

Palha, N, Guivel-Benhassine, F, Briolat, V, Lutfalla, G, Sourisseau, M, Ellett, F, Wang, C-H, Lieschke, G, Herbomel, P, Schwartz, O & Levraud, J-P 2013, 'Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish', PLoS Pathogens, vol. 9, no. 9 (Art. No.: e1003619), pp. 1 - 15. https://doi.org/10.1371/journal.ppat.1003619

Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish. / Palha, Nuno; Guivel-Benhassine, Florence; Briolat, Valerie; Lutfalla, Georges; Sourisseau, Marion; Ellett, Felix; Wang, Chieh-Huei; Lieschke, Graham; Herbomel, Philippe; Schwartz, Olivier; Levraud, Jean-Pierre.

In: PLoS Pathogens, Vol. 9, No. 9 (Art. No.: e1003619), 2013, p. 1 - 15.

Research output: Contribution to journalArticleResearchpeer-review

TY - JOUR

T1 - Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish

AU - Palha, Nuno

AU - Guivel-Benhassine, Florence

AU - Briolat, Valerie

AU - Lutfalla, Georges

AU - Sourisseau, Marion

AU - Ellett, Felix

AU - Wang, Chieh-Huei

AU - Lieschke, Graham

AU - Herbomel, Philippe

AU - Schwartz, Olivier

AU - Levraud, Jean-Pierre

PY - 2013

Y1 - 2013

N2 - Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe a novel CHIKV infection model in zebrafish, where viral spread was live-imaged in the whole body up to cellular resolution. Infected cells emerged in various organs in one principal wave with a median appearance time of approximately 14 hours post infection. Timing of infected cell death was organ dependent, leading to a shift of CHIKV localization towards the brain. As in mammals, CHIKV infection triggered a strong type-I interferon (IFN) response, critical for survival. IFN was mainly expressed by neutrophils and hepatocytes. Cell type specific ablation experiments further demonstrated that neutrophils play a crucial, unexpected role in CHIKV containment. Altogether, our results show that the zebrafish represents a novel valuable model to dynamically visualize replication, pathogenesis and host responses to a human virus.

AB - Chikungunya Virus (CHIKV), a re-emerging arbovirus that may cause severe disease, constitutes an important public health problem. Herein we describe a novel CHIKV infection model in zebrafish, where viral spread was live-imaged in the whole body up to cellular resolution. Infected cells emerged in various organs in one principal wave with a median appearance time of approximately 14 hours post infection. Timing of infected cell death was organ dependent, leading to a shift of CHIKV localization towards the brain. As in mammals, CHIKV infection triggered a strong type-I interferon (IFN) response, critical for survival. IFN was mainly expressed by neutrophils and hepatocytes. Cell type specific ablation experiments further demonstrated that neutrophils play a crucial, unexpected role in CHIKV containment. Altogether, our results show that the zebrafish represents a novel valuable model to dynamically visualize replication, pathogenesis and host responses to a human virus.

UR - http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3764224/pdf/ppat.1003619.pdf

U2 - 10.1371/journal.ppat.1003619

DO - 10.1371/journal.ppat.1003619

M3 - Article

VL - 9

SP - 1

EP - 15

JO - PLoS Pathogens

JF - PLoS Pathogens

SN - 1553-7366

IS - 9 (Art. No.: e1003619)

ER -

Palha N, Guivel-Benhassine F, Briolat V, Lutfalla G, Sourisseau M, Ellett F et al. Real-time whole-body visualization of Chikungunya Virus infection and host interferon response in zebrafish. PLoS Pathogens. 2013;9(9 (Art. No.: e1003619)):1 - 15. https://doi.org/10.1371/journal.ppat.1003619