Real-time interactive two-photon photoconversion of recirculating lymphocytes for discontinuous cell tracking in live adult mice

Tatyana Chtanova, Henry R Hampton, Louise A Waterhouse, Katherine Wood, Michio Tomura, Yoshihiro Miwa, Charles R Mackay, Robert Brink, Tri G Phan

    Research output: Contribution to journalArticleResearchpeer-review

    34 Citations (Scopus)

    Abstract

    The potential usefulness of intravital two-photon microscopy for fate mapping is limited by its inability to track cells beyond the confines of the imaging volume. Therefore, we have developed and validated a novel method for in vivo photolabelling of spatially-restricted cells expressing the Kaede optical highlighter by two-photon excitation. This has allowed us to optically mark a cohort of follicular B cells and track their dissemination from the original imaging volume in the lymph node to the spleen and contralateral lymph node. We also present the first demonstration, to our knowledge, of in vivo photoconversion of a freely moving single cell in a live adult animal. This method of `discontinuous cell tracking therefore significantly extends the fate mapping capabilities of two-photon microscopy to delineate the spatiotemporal dynamics of cellular processes that span multiple anatomical sites at the single cell level.
    Original languageEnglish
    Pages (from-to)425 - 433
    Number of pages9
    JournalJournal of Biophotonics
    Volume7
    Issue number6
    DOIs
    Publication statusPublished - 2014

    Cite this

    Chtanova, T., Hampton, H. R., Waterhouse, L. A., Wood, K., Tomura, M., Miwa, Y., ... Phan, T. G. (2014). Real-time interactive two-photon photoconversion of recirculating lymphocytes for discontinuous cell tracking in live adult mice. Journal of Biophotonics, 7(6), 425 - 433. https://doi.org/10.1002/jbio.201200175
    Chtanova, Tatyana ; Hampton, Henry R ; Waterhouse, Louise A ; Wood, Katherine ; Tomura, Michio ; Miwa, Yoshihiro ; Mackay, Charles R ; Brink, Robert ; Phan, Tri G. / Real-time interactive two-photon photoconversion of recirculating lymphocytes for discontinuous cell tracking in live adult mice. In: Journal of Biophotonics. 2014 ; Vol. 7, No. 6. pp. 425 - 433.
    @article{21399400904f43808f6fc7ef3df81fac,
    title = "Real-time interactive two-photon photoconversion of recirculating lymphocytes for discontinuous cell tracking in live adult mice",
    abstract = "The potential usefulness of intravital two-photon microscopy for fate mapping is limited by its inability to track cells beyond the confines of the imaging volume. Therefore, we have developed and validated a novel method for in vivo photolabelling of spatially-restricted cells expressing the Kaede optical highlighter by two-photon excitation. This has allowed us to optically mark a cohort of follicular B cells and track their dissemination from the original imaging volume in the lymph node to the spleen and contralateral lymph node. We also present the first demonstration, to our knowledge, of in vivo photoconversion of a freely moving single cell in a live adult animal. This method of `discontinuous cell tracking therefore significantly extends the fate mapping capabilities of two-photon microscopy to delineate the spatiotemporal dynamics of cellular processes that span multiple anatomical sites at the single cell level.",
    author = "Tatyana Chtanova and Hampton, {Henry R} and Waterhouse, {Louise A} and Katherine Wood and Michio Tomura and Yoshihiro Miwa and Mackay, {Charles R} and Robert Brink and Phan, {Tri G}",
    year = "2014",
    doi = "10.1002/jbio.201200175",
    language = "English",
    volume = "7",
    pages = "425 -- 433",
    journal = "Journal of Biophotonics",
    issn = "1864-063X",
    publisher = "Wiley-VCH Verlag GmbH & Co. KGaA",
    number = "6",

    }

    Real-time interactive two-photon photoconversion of recirculating lymphocytes for discontinuous cell tracking in live adult mice. / Chtanova, Tatyana; Hampton, Henry R; Waterhouse, Louise A; Wood, Katherine; Tomura, Michio; Miwa, Yoshihiro; Mackay, Charles R; Brink, Robert; Phan, Tri G.

    In: Journal of Biophotonics, Vol. 7, No. 6, 2014, p. 425 - 433.

    Research output: Contribution to journalArticleResearchpeer-review

    TY - JOUR

    T1 - Real-time interactive two-photon photoconversion of recirculating lymphocytes for discontinuous cell tracking in live adult mice

    AU - Chtanova, Tatyana

    AU - Hampton, Henry R

    AU - Waterhouse, Louise A

    AU - Wood, Katherine

    AU - Tomura, Michio

    AU - Miwa, Yoshihiro

    AU - Mackay, Charles R

    AU - Brink, Robert

    AU - Phan, Tri G

    PY - 2014

    Y1 - 2014

    N2 - The potential usefulness of intravital two-photon microscopy for fate mapping is limited by its inability to track cells beyond the confines of the imaging volume. Therefore, we have developed and validated a novel method for in vivo photolabelling of spatially-restricted cells expressing the Kaede optical highlighter by two-photon excitation. This has allowed us to optically mark a cohort of follicular B cells and track their dissemination from the original imaging volume in the lymph node to the spleen and contralateral lymph node. We also present the first demonstration, to our knowledge, of in vivo photoconversion of a freely moving single cell in a live adult animal. This method of `discontinuous cell tracking therefore significantly extends the fate mapping capabilities of two-photon microscopy to delineate the spatiotemporal dynamics of cellular processes that span multiple anatomical sites at the single cell level.

    AB - The potential usefulness of intravital two-photon microscopy for fate mapping is limited by its inability to track cells beyond the confines of the imaging volume. Therefore, we have developed and validated a novel method for in vivo photolabelling of spatially-restricted cells expressing the Kaede optical highlighter by two-photon excitation. This has allowed us to optically mark a cohort of follicular B cells and track their dissemination from the original imaging volume in the lymph node to the spleen and contralateral lymph node. We also present the first demonstration, to our knowledge, of in vivo photoconversion of a freely moving single cell in a live adult animal. This method of `discontinuous cell tracking therefore significantly extends the fate mapping capabilities of two-photon microscopy to delineate the spatiotemporal dynamics of cellular processes that span multiple anatomical sites at the single cell level.

    UR - http://onlinelibrary.wiley.com/doi/10.1002/jbio.201200175/pdf

    U2 - 10.1002/jbio.201200175

    DO - 10.1002/jbio.201200175

    M3 - Article

    VL - 7

    SP - 425

    EP - 433

    JO - Journal of Biophotonics

    JF - Journal of Biophotonics

    SN - 1864-063X

    IS - 6

    ER -