TY - JOUR
T1 - Real-time and in-situ monitoring of H2O2 release from living cells by a stretchable electrochemical biosensor based on vertically aligned gold nanowires
AU - Lyu, Quanxia
AU - Zhai, Qingfeng
AU - Dyson, Jennifer
AU - Gong, Shu
AU - Zhao, Yunmeng
AU - Ling, Yunzhi
AU - Chandrasekaran, Ramya
AU - Dong, Dashen
AU - Cheng, Wenlong
PY - 2019/11/5
Y1 - 2019/11/5
N2 - Traditional electrochemical biosensing electrodes (e.g., gold disk, glassy carbon electrode, etc.) can undergo sophisticated design to detect chemicals/biologicals from cells. However, such electrodes are typically rigid and nonstretchable, rendering it challenging to detect cellular activities in real-time and in situ when cells are in mechanically deformed states. Here, we report a new stretchable electrochemical cell-sensing platform based on vertically aligned gold nanowires embedded in PDMS (v-AuNWs/PDMS). Using H2O2 as a model analyte, we show that the v-AuNWs/PDMS electrode can display an excellent sensing performance with a wide linear range, from 40 μM to 15 mM, and a high sensitivity of 250 mA/cm2/M at a potential of -0.3 V. Moreover, living cells can grow directly on our stretchable high-surface area electrodes with strong adhesion, demonstrating their excellent biocompatibility. Further cell stimulation by adding chemicals induced H2O2 generation, which can be detected in real-time and in situ using our v-AuNWs/PDMS platform for both natural and stretched states of cells. Our results indicate the v-AuNWs/PDMS electrochemical biosensor may serve as a general cell-sensing platform for living organisms under deformed states.
AB - Traditional electrochemical biosensing electrodes (e.g., gold disk, glassy carbon electrode, etc.) can undergo sophisticated design to detect chemicals/biologicals from cells. However, such electrodes are typically rigid and nonstretchable, rendering it challenging to detect cellular activities in real-time and in situ when cells are in mechanically deformed states. Here, we report a new stretchable electrochemical cell-sensing platform based on vertically aligned gold nanowires embedded in PDMS (v-AuNWs/PDMS). Using H2O2 as a model analyte, we show that the v-AuNWs/PDMS electrode can display an excellent sensing performance with a wide linear range, from 40 μM to 15 mM, and a high sensitivity of 250 mA/cm2/M at a potential of -0.3 V. Moreover, living cells can grow directly on our stretchable high-surface area electrodes with strong adhesion, demonstrating their excellent biocompatibility. Further cell stimulation by adding chemicals induced H2O2 generation, which can be detected in real-time and in situ using our v-AuNWs/PDMS platform for both natural and stretched states of cells. Our results indicate the v-AuNWs/PDMS electrochemical biosensor may serve as a general cell-sensing platform for living organisms under deformed states.
UR - http://www.scopus.com/inward/record.url?scp=85073216396&partnerID=8YFLogxK
U2 - 10.1021/acs.analchem.9b02610
DO - 10.1021/acs.analchem.9b02610
M3 - Article
C2 - 31549803
AN - SCOPUS:85073216396
VL - 91
SP - 13521
EP - 13527
JO - Analytical Chemistry
JF - Analytical Chemistry
SN - 0003-2700
IS - 21
ER -