Real-time and in-situ monitoring of H2O2 release from living cells by a stretchable electrochemical biosensor based on vertically aligned gold nanowires

Quanxia Lyu, Qingfeng Zhai, Jennifer Dyson, Shu Gong, Yunmeng Zhao, Yunzhi Ling, Ramya Chandrasekaran, Dashen Dong, Wenlong Cheng

Research output: Contribution to journalArticleResearchpeer-review

64 Citations (Scopus)


Traditional electrochemical biosensing electrodes (e.g., gold disk, glassy carbon electrode, etc.) can undergo sophisticated design to detect chemicals/biologicals from cells. However, such electrodes are typically rigid and nonstretchable, rendering it challenging to detect cellular activities in real-time and in situ when cells are in mechanically deformed states. Here, we report a new stretchable electrochemical cell-sensing platform based on vertically aligned gold nanowires embedded in PDMS (v-AuNWs/PDMS). Using H2O2 as a model analyte, we show that the v-AuNWs/PDMS electrode can display an excellent sensing performance with a wide linear range, from 40 μM to 15 mM, and a high sensitivity of 250 mA/cm2/M at a potential of -0.3 V. Moreover, living cells can grow directly on our stretchable high-surface area electrodes with strong adhesion, demonstrating their excellent biocompatibility. Further cell stimulation by adding chemicals induced H2O2 generation, which can be detected in real-time and in situ using our v-AuNWs/PDMS platform for both natural and stretched states of cells. Our results indicate the v-AuNWs/PDMS electrochemical biosensor may serve as a general cell-sensing platform for living organisms under deformed states.

Original languageEnglish
Pages (from-to)13521-13527
Number of pages7
JournalAnalytical Chemistry
Issue number21
Publication statusPublished - 5 Nov 2019

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