RCAN 1 and 3 proteins regulate thymic positive selection

Eva Serrano-Candelas, German R Aleman-Muench, Sonia Sole-Sanchez, Anna Aubareda, Sergio Martinez-Hoyer, Jaume Adan, Alvaro Aranguren-Ibanez, Melanie A Pritchard, Gloria Soldevila, Merce Perez-Riba

Research output: Contribution to journalArticleResearchpeer-review

1 Citation (Scopus)

Abstract

Cooperation between calcineurin (CN)-NFATc and RAF-MEK-ERK signaling pathways is essential in thymocyte positive selection. It is known that the Regulators of Calcineurin (RCAN) proteins can act either facilitating or suppressing CN-dependent signaling events. Here, we show that RCAN genes are expressed in lymphoid tissues, and address the role of RCAN proteins in T cell development. Overexpression of human RCAN3 and RCAN1 can modulate T cell development by increasing positive selection-related surface markers, as well as the Erk(hi) competence state in double positive thymocytes, a characteristic molecular signature of positive selection, without affecting CN activity. We also found that RCAN1/3 interact with RAF kinases and CN in a non-exclusive manner. Our data suggests that the balance of RCAN interactions with CN and/or RAF kinases may influence T cell positive selection.
Original languageEnglish
Pages (from-to)295-301
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume460
Issue number2
DOIs
Publication statusPublished - 2015

Keywords

  • Calcineurin
  • NFATc
  • RAF kinase
  • RCAN1
  • RCAN3
  • Thymocyte positive selection

Cite this

Serrano-Candelas, E., Aleman-Muench, G. R., Sole-Sanchez, S., Aubareda, A., Martinez-Hoyer, S., Adan, J., ... Perez-Riba, M. (2015). RCAN 1 and 3 proteins regulate thymic positive selection. Biochemical and Biophysical Research Communications, 460(2), 295-301. https://doi.org/10.1016/j.bbrc.2015.03.029
Serrano-Candelas, Eva ; Aleman-Muench, German R ; Sole-Sanchez, Sonia ; Aubareda, Anna ; Martinez-Hoyer, Sergio ; Adan, Jaume ; Aranguren-Ibanez, Alvaro ; Pritchard, Melanie A ; Soldevila, Gloria ; Perez-Riba, Merce. / RCAN 1 and 3 proteins regulate thymic positive selection. In: Biochemical and Biophysical Research Communications. 2015 ; Vol. 460, No. 2. pp. 295-301.
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keywords = "Calcineurin, NFATc, RAF kinase, RCAN1, RCAN3, Thymocyte positive selection",
author = "Eva Serrano-Candelas and Aleman-Muench, {German R} and Sonia Sole-Sanchez and Anna Aubareda and Sergio Martinez-Hoyer and Jaume Adan and Alvaro Aranguren-Ibanez and Pritchard, {Melanie A} and Gloria Soldevila and Merce Perez-Riba",
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Serrano-Candelas, E, Aleman-Muench, GR, Sole-Sanchez, S, Aubareda, A, Martinez-Hoyer, S, Adan, J, Aranguren-Ibanez, A, Pritchard, MA, Soldevila, G & Perez-Riba, M 2015, 'RCAN 1 and 3 proteins regulate thymic positive selection', Biochemical and Biophysical Research Communications, vol. 460, no. 2, pp. 295-301. https://doi.org/10.1016/j.bbrc.2015.03.029

RCAN 1 and 3 proteins regulate thymic positive selection. / Serrano-Candelas, Eva; Aleman-Muench, German R; Sole-Sanchez, Sonia; Aubareda, Anna; Martinez-Hoyer, Sergio; Adan, Jaume; Aranguren-Ibanez, Alvaro; Pritchard, Melanie A; Soldevila, Gloria; Perez-Riba, Merce.

In: Biochemical and Biophysical Research Communications, Vol. 460, No. 2, 2015, p. 295-301.

Research output: Contribution to journalArticleResearchpeer-review

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AU - Serrano-Candelas, Eva

AU - Aleman-Muench, German R

AU - Sole-Sanchez, Sonia

AU - Aubareda, Anna

AU - Martinez-Hoyer, Sergio

AU - Adan, Jaume

AU - Aranguren-Ibanez, Alvaro

AU - Pritchard, Melanie A

AU - Soldevila, Gloria

AU - Perez-Riba, Merce

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AB - Cooperation between calcineurin (CN)-NFATc and RAF-MEK-ERK signaling pathways is essential in thymocyte positive selection. It is known that the Regulators of Calcineurin (RCAN) proteins can act either facilitating or suppressing CN-dependent signaling events. Here, we show that RCAN genes are expressed in lymphoid tissues, and address the role of RCAN proteins in T cell development. Overexpression of human RCAN3 and RCAN1 can modulate T cell development by increasing positive selection-related surface markers, as well as the Erk(hi) competence state in double positive thymocytes, a characteristic molecular signature of positive selection, without affecting CN activity. We also found that RCAN1/3 interact with RAF kinases and CN in a non-exclusive manner. Our data suggests that the balance of RCAN interactions with CN and/or RAF kinases may influence T cell positive selection.

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Serrano-Candelas E, Aleman-Muench GR, Sole-Sanchez S, Aubareda A, Martinez-Hoyer S, Adan J et al. RCAN 1 and 3 proteins regulate thymic positive selection. Biochemical and Biophysical Research Communications. 2015;460(2):295-301. https://doi.org/10.1016/j.bbrc.2015.03.029