Rat model of spinal cord injury preserving dura mater integrity and allowing measurements of cerebrospinal fluid pressure and spinal cord blood flow

Marc Soubeyrand; Elisabeth Laemmel; Charles Court; Arnaud Dubory; Eric Vicaut; Jacques Duranteau

doi:10.1007/s00586-013-2744-2

Rat model of spinal cord injury preserving dura mater integrity and allowing measurements of cerebrospinal fluid pressure and spinal cord blood flow

Marc Soubeyrand, Elisabeth Laemmel, Charles Court, Arnaud Dubory, Eric Vicaut, Jacques Duranteau

Research output: Contribution to journal › Article › Research › peer-review

19 Citations (Scopus)

Abstract

Purposes: Cerebrospinal fluid (CSF) pressure elevation may worsen spinal cord ischaemia after spinal cord injury (SCI). We developed a rat model to investigate relationships between CSF pressure and spinal cord blood flow (SCBF). Methods: Male Wistar rats had SCI induced at Th10 (n = 7) or a sham operation (n = 10). SCBF was measured using laser-Doppler and CSF pressure via a sacral catheter. Dural integrity was assessed using subdural methylene-blue injection (n = 5) and myelography (n = 5). Results: The SCI group had significantly lower SCBF (p < 0.0001) and higher CSF pressure (p < 0.0001) values compared to the sham-operated group. Sixty minutes after SCI or sham operation, CSF pressure was 8.6 ± 0.4 mmHg in the SCI group versus 5.5 ± 0.5 mmHg in the sham-operated group. No dural tears were found after SCI. Conclusion: Our rat model allows SCBF and CSF pressure measurements after induced SCI. After SCI, CSF pressure significantly increases.

Original language	English
Pages (from-to)	1810-1819
Number of pages	10
Journal	European Spine Journal
Volume	22
Issue number	8
DOIs	https://doi.org/10.1007/s00586-013-2744-2
Publication status	Published - Aug 2013
Externally published	Yes

Keywords

Cerebrospinal fluid pressure
Ischaemia
Regional blood flow
Spinal cord blood flow
Spinal cord injury

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10.1007/s00586-013-2744-2

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title = "Rat model of spinal cord injury preserving dura mater integrity and allowing measurements of cerebrospinal fluid pressure and spinal cord blood flow",

abstract = "Purposes: Cerebrospinal fluid (CSF) pressure elevation may worsen spinal cord ischaemia after spinal cord injury (SCI). We developed a rat model to investigate relationships between CSF pressure and spinal cord blood flow (SCBF). Methods: Male Wistar rats had SCI induced at Th10 (n = 7) or a sham operation (n = 10). SCBF was measured using laser-Doppler and CSF pressure via a sacral catheter. Dural integrity was assessed using subdural methylene-blue injection (n = 5) and myelography (n = 5). Results: The SCI group had significantly lower SCBF (p < 0.0001) and higher CSF pressure (p < 0.0001) values compared to the sham-operated group. Sixty minutes after SCI or sham operation, CSF pressure was 8.6 ± 0.4 mmHg in the SCI group versus 5.5 ± 0.5 mmHg in the sham-operated group. No dural tears were found after SCI. Conclusion: Our rat model allows SCBF and CSF pressure measurements after induced SCI. After SCI, CSF pressure significantly increases.",

keywords = "Cerebrospinal fluid pressure, Ischaemia, Regional blood flow, Spinal cord blood flow, Spinal cord injury",

author = "Marc Soubeyrand and Elisabeth Laemmel and Charles Court and Arnaud Dubory and Eric Vicaut and Jacques Duranteau",

year = "2013",

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doi = "10.1007/s00586-013-2744-2",

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T1 - Rat model of spinal cord injury preserving dura mater integrity and allowing measurements of cerebrospinal fluid pressure and spinal cord blood flow

AU - Soubeyrand, Marc

AU - Laemmel, Elisabeth

AU - Court, Charles

AU - Dubory, Arnaud

AU - Vicaut, Eric

AU - Duranteau, Jacques

PY - 2013/8

Y1 - 2013/8

N2 - Purposes: Cerebrospinal fluid (CSF) pressure elevation may worsen spinal cord ischaemia after spinal cord injury (SCI). We developed a rat model to investigate relationships between CSF pressure and spinal cord blood flow (SCBF). Methods: Male Wistar rats had SCI induced at Th10 (n = 7) or a sham operation (n = 10). SCBF was measured using laser-Doppler and CSF pressure via a sacral catheter. Dural integrity was assessed using subdural methylene-blue injection (n = 5) and myelography (n = 5). Results: The SCI group had significantly lower SCBF (p < 0.0001) and higher CSF pressure (p < 0.0001) values compared to the sham-operated group. Sixty minutes after SCI or sham operation, CSF pressure was 8.6 ± 0.4 mmHg in the SCI group versus 5.5 ± 0.5 mmHg in the sham-operated group. No dural tears were found after SCI. Conclusion: Our rat model allows SCBF and CSF pressure measurements after induced SCI. After SCI, CSF pressure significantly increases.

AB - Purposes: Cerebrospinal fluid (CSF) pressure elevation may worsen spinal cord ischaemia after spinal cord injury (SCI). We developed a rat model to investigate relationships between CSF pressure and spinal cord blood flow (SCBF). Methods: Male Wistar rats had SCI induced at Th10 (n = 7) or a sham operation (n = 10). SCBF was measured using laser-Doppler and CSF pressure via a sacral catheter. Dural integrity was assessed using subdural methylene-blue injection (n = 5) and myelography (n = 5). Results: The SCI group had significantly lower SCBF (p < 0.0001) and higher CSF pressure (p < 0.0001) values compared to the sham-operated group. Sixty minutes after SCI or sham operation, CSF pressure was 8.6 ± 0.4 mmHg in the SCI group versus 5.5 ± 0.5 mmHg in the sham-operated group. No dural tears were found after SCI. Conclusion: Our rat model allows SCBF and CSF pressure measurements after induced SCI. After SCI, CSF pressure significantly increases.

KW - Cerebrospinal fluid pressure

KW - Ischaemia

KW - Regional blood flow

KW - Spinal cord blood flow

KW - Spinal cord injury

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DO - 10.1007/s00586-013-2744-2

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AN - SCOPUS:84881545982

SN - 0940-6719

VL - 22

SP - 1810

EP - 1819

JO - European Spine Journal

JF - European Spine Journal

IS - 8

ER -

Rat model of spinal cord injury preserving dura mater integrity and allowing measurements of cerebrospinal fluid pressure and spinal cord blood flow

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Keywords

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