Rat model of spinal cord injury preserving dura mater integrity and allowing measurements of cerebrospinal fluid pressure and spinal cord blood flow

Marc Soubeyrand, Elisabeth Laemmel, Charles Court, Arnaud Dubory, Eric Vicaut, Jacques Duranteau

Research output: Contribution to journalArticleResearchpeer-review

11 Citations (Scopus)


Purposes: Cerebrospinal fluid (CSF) pressure elevation may worsen spinal cord ischaemia after spinal cord injury (SCI). We developed a rat model to investigate relationships between CSF pressure and spinal cord blood flow (SCBF). Methods: Male Wistar rats had SCI induced at Th10 (n = 7) or a sham operation (n = 10). SCBF was measured using laser-Doppler and CSF pressure via a sacral catheter. Dural integrity was assessed using subdural methylene-blue injection (n = 5) and myelography (n = 5). Results: The SCI group had significantly lower SCBF (p < 0.0001) and higher CSF pressure (p < 0.0001) values compared to the sham-operated group. Sixty minutes after SCI or sham operation, CSF pressure was 8.6 ± 0.4 mmHg in the SCI group versus 5.5 ± 0.5 mmHg in the sham-operated group. No dural tears were found after SCI. Conclusion: Our rat model allows SCBF and CSF pressure measurements after induced SCI. After SCI, CSF pressure significantly increases.

Original languageEnglish
Pages (from-to)1810-1819
Number of pages10
JournalEuropean Spine Journal
Issue number8
Publication statusPublished - Aug 2013
Externally publishedYes


  • Cerebrospinal fluid pressure
  • Ischaemia
  • Regional blood flow
  • Spinal cord blood flow
  • Spinal cord injury

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