Rapid screening of purification strategies for the capture of a human recombinant F(ab')2 expressed in baculovirus-infected cells using a micro-plate approach and SELDI-MS

J Pezzini; V Brenac Brochier; M P Barrouillet; M Cerruti; G Clofent-Sanchez; A Schapman; A Topol; Remy Robert; C Cabanne; P Cerruti; X Santarelli

doi:10.1016/j.chromb.2009.04.034

Rapid screening of purification strategies for the capture of a human recombinant F(ab')2 expressed in baculovirus-infected cells using a micro-plate approach and SELDI-MS

J Pezzini, V Brenac Brochier, M P Barrouillet, M Cerruti, G Clofent-Sanchez, A Schapman, A Topol, Remy Robert, C Cabanne, P Cerruti, X Santarelli

Research output: Contribution to journal › Article › Research › peer-review

6 Citations (Scopus)

Abstract

The development of a capture step of a human recombinant F(ab )(2) produced and expressed in baculovirus-infected cells was investigated by screening three mixed-mode chromatography sorbents (HEA HyperCel, PPA HyperCel and MEP HyperCel) and two ion exchangers (Q Ceramic HyperD F, S Ceramic HyperD F) sorbents using a 96-well plate format and SELDI-MS. HEA HyperCel gave the best separation performance therefore the conditions tested in micro-plate were transferred to laboratory scale chromatographic experiments, confirming that the recombinant F(ab )(2) was effectively captured on the mixed-mode sorbent without any pre-treatment of the crude extract with a 82 recovery and a 39-fold purification.

Original language	English
Pages (from-to)	2428 - 2434
Number of pages	7
Journal	Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume	877
Issue number	24
DOIs	https://doi.org/10.1016/j.chromb.2009.04.034
Publication status	Published - 2009
Externally published	Yes

Access to Document

10.1016/j.chromb.2009.04.034

RM sElocation

Cite this

Pezzini, J., Brenac Brochier, V., Barrouillet, M. P., Cerruti, M., Clofent-Sanchez, G., Schapman, A., Topol, A., Robert, R., Cabanne, C., Cerruti, P., & Santarelli, X. (2009). Rapid screening of purification strategies for the capture of a human recombinant F(ab')2 expressed in baculovirus-infected cells using a micro-plate approach and SELDI-MS. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, 877(24), 2428 - 2434. https://doi.org/10.1016/j.chromb.2009.04.034

Pezzini, J ; Brenac Brochier, V ; Barrouillet, M P ; Cerruti, M ; Clofent-Sanchez, G ; Schapman, A ; Topol, A ; Robert, Remy ; Cabanne, C ; Cerruti, P ; Santarelli, X. / Rapid screening of purification strategies for the capture of a human recombinant F(ab')2 expressed in baculovirus-infected cells using a micro-plate approach and SELDI-MS. In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2009 ; Vol. 877, No. 24. pp. 2428 - 2434.

@article{741132e82bb74e32b78fdf394a5f4e84,

title = "Rapid screening of purification strategies for the capture of a human recombinant F(ab')2 expressed in baculovirus-infected cells using a micro-plate approach and SELDI-MS",

abstract = "The development of a capture step of a human recombinant F(ab )(2) produced and expressed in baculovirus-infected cells was investigated by screening three mixed-mode chromatography sorbents (HEA HyperCel, PPA HyperCel and MEP HyperCel) and two ion exchangers (Q Ceramic HyperD F, S Ceramic HyperD F) sorbents using a 96-well plate format and SELDI-MS. HEA HyperCel gave the best separation performance therefore the conditions tested in micro-plate were transferred to laboratory scale chromatographic experiments, confirming that the recombinant F(ab )(2) was effectively captured on the mixed-mode sorbent without any pre-treatment of the crude extract with a 82 recovery and a 39-fold purification.",

author = "J Pezzini and {Brenac Brochier}, V and Barrouillet, {M P} and M Cerruti and G Clofent-Sanchez and A Schapman and A Topol and Remy Robert and C Cabanne and P Cerruti and X Santarelli",

year = "2009",

doi = "10.1016/j.chromb.2009.04.034",

language = "English",

volume = "877",

pages = "2428 -- 2434",

journal = "Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences",

issn = "1570-0232",

publisher = "Elsevier",

number = "24",

}

Pezzini, J, Brenac Brochier, V, Barrouillet, MP, Cerruti, M, Clofent-Sanchez, G, Schapman, A, Topol, A, Robert, R, Cabanne, C, Cerruti, P & Santarelli, X 2009, 'Rapid screening of purification strategies for the capture of a human recombinant F(ab')2 expressed in baculovirus-infected cells using a micro-plate approach and SELDI-MS', Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, vol. 877, no. 24, pp. 2428 - 2434. https://doi.org/10.1016/j.chromb.2009.04.034

Rapid screening of purification strategies for the capture of a human recombinant F(ab')2 expressed in baculovirus-infected cells using a micro-plate approach and SELDI-MS. / Pezzini, J; Brenac Brochier, V; Barrouillet, M P; Cerruti, M; Clofent-Sanchez, G; Schapman, A; Topol, A; Robert, Remy; Cabanne, C; Cerruti, P; Santarelli, X.

In: Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences, Vol. 877, No. 24, 2009, p. 2428 - 2434.

Research output: Contribution to journal › Article › Research › peer-review

TY - JOUR

T1 - Rapid screening of purification strategies for the capture of a human recombinant F(ab')2 expressed in baculovirus-infected cells using a micro-plate approach and SELDI-MS

AU - Pezzini, J

AU - Brenac Brochier, V

AU - Barrouillet, M P

AU - Cerruti, M

AU - Clofent-Sanchez, G

AU - Schapman, A

AU - Topol, A

AU - Robert, Remy

AU - Cabanne, C

AU - Cerruti, P

AU - Santarelli, X

PY - 2009

Y1 - 2009

N2 - The development of a capture step of a human recombinant F(ab )(2) produced and expressed in baculovirus-infected cells was investigated by screening three mixed-mode chromatography sorbents (HEA HyperCel, PPA HyperCel and MEP HyperCel) and two ion exchangers (Q Ceramic HyperD F, S Ceramic HyperD F) sorbents using a 96-well plate format and SELDI-MS. HEA HyperCel gave the best separation performance therefore the conditions tested in micro-plate were transferred to laboratory scale chromatographic experiments, confirming that the recombinant F(ab )(2) was effectively captured on the mixed-mode sorbent without any pre-treatment of the crude extract with a 82 recovery and a 39-fold purification.

AB - The development of a capture step of a human recombinant F(ab )(2) produced and expressed in baculovirus-infected cells was investigated by screening three mixed-mode chromatography sorbents (HEA HyperCel, PPA HyperCel and MEP HyperCel) and two ion exchangers (Q Ceramic HyperD F, S Ceramic HyperD F) sorbents using a 96-well plate format and SELDI-MS. HEA HyperCel gave the best separation performance therefore the conditions tested in micro-plate were transferred to laboratory scale chromatographic experiments, confirming that the recombinant F(ab )(2) was effectively captured on the mixed-mode sorbent without any pre-treatment of the crude extract with a 82 recovery and a 39-fold purification.

UR - http://www.sciencedirect.com/science/article/pii/S1570023209003018

U2 - 10.1016/j.chromb.2009.04.034

DO - 10.1016/j.chromb.2009.04.034

M3 - Article

VL - 877

SP - 2428

EP - 2434

JO - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

JF - Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

SN - 1570-0232

IS - 24

ER -

Pezzini J, Brenac Brochier V, Barrouillet MP, Cerruti M, Clofent-Sanchez G, Schapman A et al. Rapid screening of purification strategies for the capture of a human recombinant F(ab')2 expressed in baculovirus-infected cells using a micro-plate approach and SELDI-MS. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2009;877(24):2428 - 2434. https://doi.org/10.1016/j.chromb.2009.04.034