The development of a capture step of a human recombinant F(ab )(2) produced and expressed in baculovirus-infected cells was investigated by screening three mixed-mode chromatography sorbents (HEA HyperCel, PPA HyperCel and MEP HyperCel) and two ion exchangers (Q Ceramic HyperD F, S Ceramic HyperD F) sorbents using a 96-well plate format and SELDI-MS. HEA HyperCel gave the best separation performance therefore the conditions tested in micro-plate were transferred to laboratory scale chromatographic experiments, confirming that the recombinant F(ab )(2) was effectively captured on the mixed-mode sorbent without any pre-treatment of the crude extract with a 82 recovery and a 39-fold purification.
|Pages (from-to)||2428 - 2434|
|Number of pages||7|
|Journal||Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences|
|Publication status||Published - 2009|