Rapid screening of purification strategies for the capture of a human recombinant F(ab')2 expressed in baculovirus-infected cells using a micro-plate approach and SELDI-MS

J Pezzini, V Brenac Brochier, M P Barrouillet, M Cerruti, G Clofent-Sanchez, A Schapman, A Topol, Remy Robert, C Cabanne, P Cerruti, X Santarelli

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6 Citations (Scopus)

Abstract

The development of a capture step of a human recombinant F(ab )(2) produced and expressed in baculovirus-infected cells was investigated by screening three mixed-mode chromatography sorbents (HEA HyperCel, PPA HyperCel and MEP HyperCel) and two ion exchangers (Q Ceramic HyperD F, S Ceramic HyperD F) sorbents using a 96-well plate format and SELDI-MS. HEA HyperCel gave the best separation performance therefore the conditions tested in micro-plate were transferred to laboratory scale chromatographic experiments, confirming that the recombinant F(ab )(2) was effectively captured on the mixed-mode sorbent without any pre-treatment of the crude extract with a 82 recovery and a 39-fold purification.
Original languageEnglish
Pages (from-to)2428 - 2434
Number of pages7
JournalJournal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
Volume877
Issue number24
DOIs
Publication statusPublished - 2009
Externally publishedYes

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